© 2004 BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine
SHORT REPORT
The validity of capillary blood sampling in the determination of human growth hormone concentration during exercise in men
1 Sport Sciences, Brunel University, Uxbridge, UK
2 BOMC, Northwick Park, Harrow, UK
3 St Martin’s College, Lancaster, UK
4 School of Sport, Performing Arts and Leisure, Wolverhampton University
5 School of Engineering, Cranfield University of Technology, Cranfield, Bedfordshire, UK
Correspondence to:
Correspondence to:
R J Godfrey
Brunel University, Uxbridge, Middlesex UB8 3PH, UK; richard.godfrey{at}brunel.ac.uk
Background: Studies measuring human growth hormone (hGH) in blood during exercise have mainly used venous sampling. The invasive nature of this procedure makes evaluation of hGH impossible in various exercise environments.
Objective: To determine whether capillary sampling could offer an alternative sampling method.
Methods: Capillary and venous blood samples were collected for determination of hGH at the end of each exercise stage during an incremental exercise test in 16 male club level competitive cyclists (mean (SD) age 30.8 (8.0) years, body mass 72.2 (7.1) kg, body fat 12.9 (3.5)%, peak oxygen consumption 4.18 (0.46) l•min–1). Linear regression, from a plot of venous v capillary blood hGH concentration, showed a correlation coefficient of r = 0.986 (p<0.001). When geometric means and log transformations were used, a coefficient of variation of 14.2% was demonstrated between venous and capillary flow for hGH concentration. The mean ratio limits of agreement were 0.62 (1.72)—that is, 95% of the ratios were contained between 0.36 and 1.07, with a mean of 0.62.
Conclusions: Capillary blood sampling is an acceptable alternative to venous sampling for determining hGH concentration during rest and exercise. Sample sites should not be used interchangeably: one site should be chosen and its use standardised.
Abbreviations: FFA, free fatty acids; hGH, human growth hormone; IRMA, immunoradiometric assay
Keywords: growth hormone measurement; sample site; exercise
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Br. J. Sports Med. 2004 38: 585.
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