© 2005 BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine
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The following electronic only articles are published in conjunction with this issue of BJSM (see also pages 253 and 297)
A Scott, K M Khan, J Heer, et al
Background: The role of apoptosis, or programmed cell death, has only recently been explored in tendon.
Objective: To investigate the development of apoptosis after high strain loading of rat tendon.
Methods: The right tibialis anterior tendons of three rats were prepared for mechanical loading, and left tendons were prepared identically as non-loaded controls. Tendon was loaded with 20% strain for six hours using a 1 Hz longitudinal sine wave signal. The following were used to assess apoptosis: (a) a monoclonal mouse antibody (F7-26) to label single stranded DNA breaks; (b) a rabbit polyclonal antibody that specifically recognises the cleaved form of caspase-3.
Results: Light microscopy confirmed that the high strain protocol induced a stretch overload injury. Control tendons showed little
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