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British Journal of Sports Medicine 2005;39:266
Copyright © 2005 BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine.
Br J Sports Med 2005;39:266
© 2005 BMJ Publishing Group Ltd & British Association of Sport and Exercise Medicine

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The first 150 words of the full text of this article appear below.

The following electronic only articles are published in conjunction with this issue of BJSM (see also pages 253 and 297)

High strain mechanical loading rapidly induces tendon apoptosis: an ex vivo rat tibialis anterior model

A Scott, K M Khan, J Heer, et al

Background: The role of apoptosis, or programmed cell death, has only recently been explored in tendon.

Objective: To investigate the development of apoptosis after high strain loading of rat tendon.

Methods: The right tibialis anterior tendons of three rats were prepared for mechanical loading, and left tendons were prepared identically as non-loaded controls. Tendon was loaded with 20% strain for six hours using a 1 Hz longitudinal sine wave signal. The following were used to assess apoptosis: (a) a monoclonal mouse antibody (F7-26) to label single stranded DNA breaks; (b) a rabbit polyclonal antibody that specifically recognises the cleaved form of caspase-3.

Results: Light microscopy confirmed that the high strain protocol induced a stretch overload injury. Control tendons showed little . . . [Full text of this article]


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