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Left ventricular systolic function and diastolic filling after intermittent high intensity team sports
  1. K P George1,
  2. E Dawson2,
  3. R E Shave3,
  4. G Whyte3,
  5. M Jones2,
  6. E Hare2,
  7. D Gaze4,
  8. P Collinson4
  1. 1Liverpool John Moores University, Liverpool, UK
  2. 2Manchester Metropolitan University, Manchester, UK
  3. 3British Olympic Medical Centre, Northwick Park Hospital, Harrow, Middlesex, UK
  4. 4St George’s, London, UK
  1. Correspondence to:
 Dr George
 Centre for Sport and Exercise Science, Liverpool John Moores University, Trueman Street, Liverpool L3 2ET, UK; k.georgelivjm.ac.uk

Abstract

Background: Prolonged steady state exercise can lead to a decrease in left ventricular (LV) function as well as promote the release of cardiac troponin T (cTnT). There is limited information on the effect of intermittent high intensity exercise of moderate duration.

Objectives: To determine the effect of intermittent high intensity exercise of moderate duration on LV function.

Methods: Nineteen male rugby and football players (mean (SD) age 21 (2) years) volunteered. Assessments, before, immediately after, and 24 hours after competitive games, included body mass, heart rate (HR), and systolic blood pressure (sBP) as well as echocardiography to assess stroke volume (SV), ejection fraction (EF), systolic blood pressure/end systolic volume ratio (sBP/ESV), and global diastolic filling (E:A) as well as to indirectly quantify preload (LV internal dimension at end diastole (LVIDd)). Serum cTnT was analysed using a 3rd generation assay. Changes in LV function were analysed by repeated measures analysis of variance. cTnT data are presented descriptively.

Results: SV (91 (26) v 91 (36) v 90 (35) ml before, after, and 24 hours after the game respectively), EF (71 (8) v 70 (9) v 71 (7)%), and sBP/ESV (4.2 (1.8) v 3.8 (1.9) v 4.1 (1.6) mm Hg/ml) were not significantly altered (p>0.05). Interestingly, whereas LVIDd was maintained after the game (50 (5) v 50 (6) mm), sBP was transiently but significantly reduced (131 (3) v 122 (3) mm Hg; p<0.05). E:A was moderately (p<0.05) reduced after the game (2.0 (0.4) v 1.5 (0.4)) but returned to baseline within 24 hours. No blood sample contained detectable levels of cTnT.

Conclusions: In this cohort, LV systolic function was not significantly altered after intermittent activity. A transient depression in global diastolic filling was partially attributable to a raised HR and could not be explained by myocyte disruption as represented by cTnT release.

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Despite some contradictory findings, current evidence suggests that prolonged steady state exercise results in a reduction in left ventricular (LV) systolic and diastolic filling as well as occasionally promoting the appearance of cardiac specific troponins (cTnT), normally indicative of myocyte necrosis.1,2 Evidence of such depression in LV function and appearance of cTnT is generally transient3,4 and thus the term “exercise induced cardiac fatigue” (EICF) has been promoted.2 The impact on performance, the clinical consequences, and the causative factors underpinning EICF have yet to be clarified, as has the full description of the exercise circumstances that will potentially precipitate EICF.

To date most of the available EICF literature is on young, well trained athletes competing in a range of ultraendurance activities up to 24 hours in duration.4–14 Limited data are available for shorter durations (90–120 minutes) of prolonged exercise, and the few studies available are laboratory based and have produced contentious results.15–19 Owing to the extended duration of activities reported in previous studies, this type of exercise tends to be relatively submaximal and steady state in nature. Such events contain very limited periods of intense cardiovascular acceleration and/or deceleration and few or no resistive/isometric components that may increase myocardial oxygen demand.

To our knowledge, there are no data for young healthy subjects taking part in moderate duration exercise with intermittent periods of explosive, high intensity, and isometric muscle activity. Although we have some evidence that short term (15 minutes), intermittent, high intensity activity19 and one set of resistance exercises20,21 does not reduce LV contractile function, we do not know the consequences of longer sessions of intermittent, high intensity exercise beyond the duration that has reported depressed LV function with steady state exercise.15–17 Because of the known consequences for blood pressure of isometric activity in major muscle groups22 as well as the sustained high heart rates,23 intermittent team game activities with periods of high intensity work combined with isometric muscle contractions may result in a depression in LV function after exercise and the appearance of cTnT in the same way as prolonged steady state activity.

With the high levels of participation in team sports and in the light of developing cTnT technology24 and debate about the clinical significance of cTnT in non-pathological populations,25 it is pertinent to investigate the broad concept of EICF in such circumstances. Therefore we investigated LV function and cTnT before and after common team games (rugby and football) that include a significant high intensity and/or isometric muscle contraction component.

METHODS

Subjects and design

Nineteen male subjects (mean (SD) age 21 (2) years; nine rugby league and 10 soccer players competing in interuniversity games) provided written informed consent. Exclusion criteria included any personal or early family history of cardiopulmonary disease. Ethical approval was provided by Manchester Metropolitan University.

The study used a repeated measures design. The first echocardiographic assessment took place about 24 hours before the designated game. Immediately after the game (within 30 minutes of its completion before showering and fluid replacement), subjects were assessed again. They then returned 24 hours after the game for the final assessment. Subjects were recruited for a range of games (maximum two players for any given game) and were excluded if they had been substituted at any point of the game. Because of the multiple number of games in which the players competed, no control was placed on the nature of the opposition or the ambient conditions. An attempt to determine heart rate (HR) during games by telemetry was logistically difficult, and we resorted to a reflective interpretation of match analysis literature that suggested that both soccer and rugby included significant periods of high intensity activity interspersed with spells of low intensity (recovery) activity.22 We recorded ambient temperature and relative humidity at the assessment immediately after the game, and this varied between games (temperature range 4–12°C; humidity range 26–54%). All subjects were advised to abstain from hard training in the 24 hours before and after the game. They were advised not to consume alcohol or caffeine for a minimum of six hours before any assessment.

Procedures

At an initial fitness and familiarisation session, all subjects completed a general health questionnaire and were then assessed for body composition and maximum oxygen uptake (V˙o2max). Body composition was determined by anthropometry using the four site skinfold equation of Durnin and Womersley.25o2max was determined by a continuous and progressive running treadmill exercise test using an Oxycon Alpha Sprint (Jaeger, Mijnhard bv, the Netherlands) online system.

At the main testing sessions, subjects were initially assessed for body mass (BM), with footwear, shirts, and shorts removed, on standard scales (Seca). Subjects then lay supine, and, after a 15 minute rest period, duplicate brachial artery systolic (sBP) and diastolic (dBP) blood pressures were assessed by standard auscultation. At this time, HR was recorded from the electrocardiograph within the echocardiography system. Each subject then underwent a two dimensionally guided M mode and Doppler echocardiographic examination in the left lateral decubitas position. This was conducted using an HP Sonos 1000 ultrasound imaging system with an integrated electrocardiograph (Hewlett-Packard, Andover, Massachusetts, USA). Using a 2.5 MHz transducer, standard two dimensionally guided M-Mode echocardiography was used to evaluate LV structures according to ASE guidelines.26 LV posterior free wall during diastole (LVPWd) and systole (LVPWs) was measured. Assessment of LV internal diameter during diastole (LVIDd) and systole (LVIDs) facilitated assessment of systolic functional indices. Ejection fraction (EF) and stroke volume (SV) were derived from M mode data using the following formulae27:

EF (%)  =  ((LVIDd3– LVIDs3)×100)/LVIDd3

SV (ml)  =  LVIDd3– LVIDs3

End systolic volume (ESV) of the LV was calculated as the cube of LVIDs, and thus the functional variable sBP/ESV was calculated due to its independence from LV loading.28

Doppler echocardiography was used to assess global diastolic filling. An apical four chamber view was imaged. Pulsed wave Doppler interrogation of mitral valve inflow velocities was then performed with alignment of the sample volume cursor parallel to flow at the level of the mitral annulus. Peak early filling (E wave, cm−1) and peak late filling (A wave, cm−1) velocities were measured, and the ratio of early to late diastolic filling (E:A) was calculated.

Because haemodynamic loading on the LV influences systolic function and diastolic filling indices, it was important to provide some indirect surrogate of both preload and afterload. In this respect LVIDd represented preload whereas LV meridonial wall stress (LVMWS) represented LV afterload29:

LVMWS (g/cm)  =  (0.334 × (sBP × LVIDs))/(LVPWs((1 + LVPWs)/LVIDs)).

One experienced sonographer was used for all examinations. Images were recorded to high quality videotape and analysed off line by a single experienced technician. The difference in HR before and after the game made it difficult to blind the technician. A minimum of three consecutive cardiac cycles were measured and averaged.

After the echocardiographic examination, 5 ml whole blood was drawn from an antecubital vein. This was allowed to clot, was centrifuged, and the serum drawn off and frozen (−20°C) for later analysis. Serum cTnT was determined using a validated 3rd generation assay30,31 by electrochemiluminescence technology used in the Elecsys 1010 automated batch analyser (Roche Diagnostics, Mannheim, Germany). Quality control data for this procedure are presented elsewhere.31

Data analysis

For data analysis, the rugby and soccer players were combined into one cohort as game duration was similar and qualitative analysis of data did not reveal any different trends between the sports. Differences in BM, sBP, dBP, HR, LV systolic and diastolic filling, and LV loading before and after the game were analysed using repeated measures one way analysis of variance, with post hoc Tukey tests where appropriate. cTnT concentrations before and after the game were descriptively analysed because of the likelihood of it not being detectable at baseline. Alterations in LV function over the game were correlated with each other, V˙o2max, HR, and LV loading conditions by Pearson’s product moment analysis. Critical α was set at 0.05, and all analyses were carried out on Statistica software (Statsoft Ltd, Tulsa, Oklahoma, USA).

RESULTS

All 19 subjects played for the entire duration of the selected games. Echocardiograms were obtained for all subjects within 30 minutes of the end of play. Despite the consumption of drinks at intervals during play, BM was significantly reduced from 75.9 (5.9) to 74.8 (6.0) kg after the game. After 24 hours, it had increased, probably as a consequence of drinking, but had not reached baseline values (75.4 (5.8) kg). Despite this reduction in BM, there was indirect evidence of a relatively stable preload because LVIDd was not changed at any assessment point (table 1). A significant reduction in sBP (about 9 mm Hg) was reported after the game, which had almost returned to baseline levels after 24 hours of recovery. However, LVMWS, a better indicator of afterload, was not significantly reduced despite a small fall (4 g/cm) during the game. Data for dBP were quite consistent over time (77.8 (1.9) v 75.9 (2.5) v 78.9 (2.6) mm Hg). HR immediately after the game was significantly raised in all subjects (about 23 beats/min) although this had returned to baseline after 24 hours.

Table 1

 Cardiovascular data for the cohort before and after a game of rugby or football

Stroke volume was maintained near baseline levels in nearly all subjects, and the mean values before and after the game were not significantly different. EF, LVPWs, and sBP/ESV—systolic functional indices more readily associated with contractility—were not different after the game. Whereas EF had decreased by about 1% immediately after the game, the reduction in LVPWs was about 4% and the decline in sBP/ESV was about 10%.

Global diastolic filling, as represented by the E:A ratio, was significantly decreased immediately after the game. Whereas changes in both E and A flow velocity contributed to the altered E:A ratio, only A was significantly different (raised) after the game. Analysis of E:A data suggested a significant correlation (r  =  −0.78, r2  =  0.61, p<0.05) between E:A and HR after the game (fig 1) but limited or no relation to other loading factors or indices of global fitness (r  =  0.13–0.31, p>0.05). Likewise there was no significant correlation between E:A and indices of LV contractility after the game (r  =  −0.07 to −0.36, p>0.05). cTnT was not detected in any blood sample.

Figure 1

Relation between heart rate and ratio of early to late diastolic filling (E:A) after the game.

DISCUSSION

This study is the first to attempt to document the potential for 80–90 minutes of intermittent, high intensity exercise to elicit characteristics of EICF. In so doing it adds to a growing database for prolonged endurance and ultraendurance activity that occurs predominantly at steady state exercise intensities.

The most obvious finding is that the field based exercise adopted did not result in any statistically significant evidence of depressed LV systolic function after the game. It is, however, interesting to note that sBP/ESV (a surrogate of LV contractility least affected by loading) was decreased by about 10% and LVPWs by about 4% after the game. EF was unaltered despite a significant reduction in sBP and a small fall in LVMWS. Whether these alterations are functionally significant, despite the lack of statistical significance, is difficult to determine, as such changes are close to, or within, measurement variability. Further research with a larger sample size, using additional indices of systolic function derived by tissue Doppler, is needed to elaborate a potential LV contractile depression.

The conservative interpretation of these data is that LV contractility was not significantly altered, probably because of the relatively well maintained preload. This is probably similar to the very short duration, high intensity exercise previously studied.20,21 The lack of significant changes in LV contractile function may also be due to the fact that the total external and cardiovascular workloads imposed were limited, despite high mean HRs and increased myocardial oxygen demand caused by short periods of isometric or high intensity work, compared with some previous ultraendurance activity.7 This is not surprising given the immense exercise volumes and effort required over the long hours of an Ironman triathlon4,7,10 or 24 hour runs.5,6 Interestingly there are few field based cardiac fatigue studies that have investigated exercise of one to two hours duration.1,2 The closest examples may be marathon running3,32–35 with race durations of three plus hours where only sporadic evidence of EICF is noted.3,34 Laboratory based studies with 60–120 minutes of steady state exercise have provided evidence to both support15–17 and refute18,19 the existence of EICF. The variability in results between studies may be due to differences in exercise intensity, subject, or technical measurements.

Whether the lack of a statistically significant depression in LV systolic function is a consequence of the relatively low level of competitive activity or the subelite nature of the cohort is impossible to determine. University players may not play at the same intensity as more elite players, and they are also unlikely to be as well trained. A continuation of this work may use different cohorts or different exercise regimens. A specific area to develop from this work may be an investigation of the consequences of hard resistance training sessions on LV function after exercise. To our knowledge, no such data are available but may be interesting given the haemodynamic pressure loading on the LV.

The decreased E:A suggests some form of depression of global diastolic filling. Although diastolic filling has received less attention than systolic function, the statistical outcome and magnitude of change in our data (2.0 to 1.5) is fairly consistent with a range of previous research.3,5,17 The reduction in E:A after the game is difficult to fully explain; however, the increase in HR after the game (significantly correlated with E:A) may account for about 60% of the variability in E:A (fig 1). Somewhat surprisingly, a raised HR after exercise could not explain the reduction in E:A reported in similar research.3,4 Other indices of LV loading measured in our study could not fully explain the remaining variability in E:A. One potential contributory factor, myocyte necrosis, can be largely discounted because of the lack of any detectable cTnT in any subject after exercise. Any further explanation for this alteration in function is therefore speculative at best, but the disturbance of intracellular Ca2+ metabolism specifically related to its removal from the cytosol has been suggested. Further research is required to clarify the relation between HR and diastolic filling after exercise and whether this is the primary contributor to the reduction in E:A reported in most cardiac fatigue studies. It is interesting that diastolic filling decreased in the absence of a depression in systolic function. Whether diastolic filling is more sensitive to the impact of prolonged and/or intermittent exercise than systolic function is again speculative but worthy of further enquiry. It is also important to note that diastolic filling is complex and poorly understood. Furthermore, it is recognised that global indices of diastolic filling (E:A) can be quite limited, and interpretation must be made with care.36 Future researchers may wish to use technologies such as tissue Doppler to obtain a more complete picture of the effect of prolonged exercise on LV diastolic filling.

The lack of cTnT in any blood sample suggests that this type of exercise does not result in myocyte necrosis or alterations in membrane permeability13 in this cohort. This suggests that myocardial stunning37 is not propagated within intermittent intensity exercise of 80–90 minutes duration. Blood cTnT concentrations after exercise have previously been assessed in steady state, field based research of duration greater than 180 minutes. Thus there are limited data with which to compare our results and the absence of cTnT. cTnT has been detected in longer studies,10,14 but this finding is by no means consistent between studies or within specific populations. The predisposing factors for cTnT appearance, and thus the clinical significance, in healthy populations performing exercise require further study. When interpreting our cTnT data, it is important to note that a potential limitation of this study was the timing of the blood sample after exercise (within 30 minutes of cessation of exercise). The time course of pathological appearance of cTnT (about four hours) may have meant that blood sampling occurred too soon to pick up the appearance of cTnT. However, if pathological release of cTnT had occurred, it would probably have still been detectable 24 hours after exercise. Membrane leakage of the cytosolic component of cTnT, as proposed by Neumayr et al,13 may also have occurred and been missed. However, this can appear substantially earlier than in scenarios such as acute myocardial infarction. Indeed there is evidence from animal models of differential temporal release patterns for cTnT depending on its origin, with membrane damage causing functionally unbound cTnT release within minutes of reperfusion in a rat heart model.38 Unpublished observations from our laboratory have reported raised cTnT within two hours of the onset of endurance exercise in humans.

In conclusion, intermittent exercise in this cohort did not result in a significant depression in LV systolic function, although further research may be valuable to confirm this. A small but significant reduction in diastolic filling after exercise could only partially be explained by HR dynamics, and further research is required to understand this change. The exercise bout did not produce any evidence of cardiac myocyte necrosis after exercise.

Take home message

Exercise bouts of moderate duration that contain intermittent periods of high intensity activity do not result in significant LV systolic functional alterations or evidence of myocyte necrosis. The alterations in LV diastolic filling after exercise are partially related to HR changes. All changes are normalised 24 hours after exercise.

REFERENCES

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