Background: Muscle fatigue has high relevance in human performance yet little research has evaluated how it should be assessed.
Objective: To perform a pilot study to identify suitable methods of generating and assessing fatigue of the trunk flexor and extensor muscles.
Methods: Sixteen university rugby players (mean (SEM) age 21.9 (0.2) years) were recruited and subjected to four protocols (A, B, C, D), separated by a week to allow recovery, with peak torque being recorded during each test: A, isokinetic measurements before and after fatigue, with a 10 repetition isokinetic fatigue period; B, isokinetic measurements before and after fatigue with a 45 second isometric fatigue period; C, isometric measurements before and after fatigue with a 10 repetition isokinetic fatigue period; D, isometric measurements before and after fatigue with a 45 second isometric fatigue period. All were conducted during flexion and extension of the trunk on the Cybex Norm Isokinetic Dynamometer trunk flexion-extension unit.
Results: All subjects completed all four protocols. Fatigue induction appeared more effective in flexion than extension. Significant differences in mean peak torque before and after fatigue were seen in protocols A, B, and D in flexion and only in protocol D for extension. In flexion, protocol D produced the greatest fatigue, peak torque being 16.2% less after than before fatigue, suggesting greatest sensitivity.
Conclusions: Protocol D, which incorporates isometric testing and fatigue protocols, appears to be able to produce fatigue most effectively, and therefore may provide the most valid assessment of fatigue in the trunk flexor and extensor muscles.
- muscle fatigue
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An often applied definition of fatigue is an “exercise-induced reduction in maximal voluntary muscle force”.1 This should not be confused with the limit of endurance, which is the time period for which a constant (non-fatiguing) force output can be maintained.2 Further confusion can arise about whether this endurance is assessed maximally or submaximally,3 and as a result of test position.
Muscle fatigue is a complex and multifaceted process involving physiological, biomechanical, and psychological elements.4 It is an important phenomenon, as there are numerous proven relations with work related musculoskeletal injuries.4,5 Being able to identify and test muscle fatigue is of importance in understanding the ergonomics and physiology of work and sport induced injury. Improved working practices and sport techniques, training, and testing can then result.4,5
Many studies of muscle fatigue have actually assessed the limit of endurance (by measuring the ability to maintain a sustained contraction), as opposed to fatigue as defined above.4,6 Consequently, very few studies have evaluated the optimal method for assessing muscle fatigue. There are two fundamental ways to measure muscle strength and therefore fatigue: isometrically and isokinetically. Isometric measurement involves contraction against a fixed, immovable object (a static test), whereas isokinetic assessment is dynamic muscular contraction at a controlled velocity (repeated contractions).7 However, it is not known which approach is optimal for firstly creating muscle fatigue and secondly quantifying it. Traditionally the Biering-Sorenson test has been used to assess fatigue of the trunk extensor muscle group.8 However, this test is not performed against maximal resistance as it is only performed against gravity. Therefore it may not be a true measure of fatigue because, according to the definition used above,1 it would not reflect a reduction in maximal voluntary muscle force and is therefore more a measure of endurance. It also may not permit a large range of performance skills to be assessed because an athlete may be capable of performing the test with ease whereas a patient with low back pain may struggle to resist gravity. It is also limited to assessment of the trunk extensor muscle group only.
The assessment of fatigue and endurance in the trunk is important because it has been widely reported that patients with low back pain develop a deconditioning syndrome that particularly influences the strength and function of the back muscles,9–16 with such patients being much weaker than healthy controls.15,17–21 Lack of endurance has also been highlighted as a key factor for predicting low back pain,22 and this suggests that the assessment of fatigue warrants further investigation. Consequently, this study focused on the flexor-extensor muscles of the spine, investigating different methods of testing and evaluating maximal fatigue of the trunk muscles.
Ethical approval for this study was obtained from the Riverside Research Ethics Committee, at Charing Cross Hospital, London, UK. Sixteen male student rugby players (mean (SEM) age 21.9 (0.2) years, weight 91.2 (3.3) kg, height 1.8 (0.03) m) were recruited from the college’s rugby club, and written informed consent was obtained. All current members of the college club who were in full training and eligible for competition were eligible for inclusion in this study, although subjects with a current or recent low back injury (time off training or intervention four weeks before testing) were excluded from taking part. All testing fell mid-season in the rugby calendar.
Four trunk fatigue protocols were tested on four separate occasions, with an interval of one week between testing, all were allowed a period of familiarisation with the equipment. The volunteers received no specific training for trunk muscle exercise before or during the study.
Tests were carried out using the Cybex Norm Isokinetic Dynamometer (Henley Healthcare, Sugarland, Texas, USA) with an incorporated trunk flexion/extension unit (fig 1). The lower limbs were stabilised by tibial and thigh pads. A belt secured the pelvis to limit the use of the hip flexors. A shoulder harness and backrest provided anchorage to the moving upper section of the apparatus. Range of motion was recorded from −10° of hyperextension to 80° flexion as recorded through the Cybex system, which represented the limits of range of the system rather than the ranges of the subjects. All isokinetic testing was performed through this range. Subjects were tested according to the following four protocols in sequential order. These protocols were derived from previous experience obtained testing elite rowers,23 but further research is required to investigate duration and repetition number.
Maximum strength before fatigue was measured for both flexion and extension during an isokinetic trunk flexion-extension test. This was performed at 60°/s and incorporated one trial followed by the test. A five second rest was used to enable the subject to prepare for the fatigue section. A series of 10 maximal isokinetic flexion-extension tests performed at 60°/s was then used to fatigue the subject. Again, another five second rest was implemented to enable preparation for testing after fatigue. Strength after fatigue was recorded in the same way as before.
Maximum strength before fatigue was measured for both flexion and extension during an isokinetic trunk flexion-extension test. This was performed at 60°/s and incorporated one trial followed by the test. A five second rest was used to enable the subject to prepare for the fatigue section. A 45 second maximal isometric hold at 10° flexion was then used to fatigue the subject. Again, another five second rest was implemented to enable preparation for testing after fatigue. Strength after fatigue was recorded in the same way as before. The above was then repeated with the fatiguing isometric hold in 10° extension.
Maximum strength before fatigue was measured during a five second isometric hold in 10° flexion. This incorporated one trial followed by the test. A five second rest was used to enable the subject to prepare for the fatigue section. A series of 10 maximal isokinetic flexion-extension tests performed at 60°/s was then used to fatigue the subject. Again, another five second rest was implemented to enable preparation for testing after fatigue. Strength after fatigue was recorded in the same way as before. The test was then repeated with the isometric holds in 10° extension.
Maximum strength before fatigue was measured during a five second isometric hold in 10° flexion. This incorporated one trial followed by the test. A five second rest was used to enable the subject to prepare for the fatigue section. A 45 second maximal isometric hold, held at 10° of flexion was then used to fatigue the subject. Again, another five second rest was implemented to enable preparation for testing after fatigue. Strength after fatigue was recorded in the same way as before. This was then repeated for extension, with the spine being held in 10° extension. Table 1 gives a summary of the protocols.
Body weight adjusted peak torque was used in the final data analyses. This was calculated as a percentage (peak torque (N.m) divided by body weight (kg) × 100). In an attempt to provide a measure of fatigue in terms of change in force output, a fatigue index was determined to quantify the change in data after fatigue. It was calculated as a percentage (value after fatigue divided by value before fatigue × 100). Thus if 100% or more is achieved, no fatigue has occurred, whereas values lower than 100 suggest that fatigue has occurred, the lower the value the greater the fatigue.
Data obtained before and after fatigue in each protocol were examined for differences using the paired Student’s t test. In addition, to investigate if a learning effect was evident within the same type of exercise for both flexion and extension, repeated measures analysis of variance was used. All four measures of peak torque under the same conditions were compared—that is, before and after values for isokinetics in protocols A and B, and before and after values for isometrics in protocols C and D. Results were considered significant when p<0.05.
All 16 subjects completed all four protocols over a four week period. Figure 2 shows the body weight adjusted mean (SEM) peak torque before and after fatigue in flexion and extension. Induction of fatigue appeared to be greater in flexion than extension. The protocols that produced a significant difference in values after fatigue were A, B, and D in flexion; only protocol D produced significant fatigue in extension. The fatigue index for protocol A in extension was 103.4%, indicating that the mean peak torque after fatigue was greater than before. Indeed, a larger proportion of subjects (9 of the 16) had an increased measurement for peak torque after the fatiguing section of the protocol than those who had a decreased measurement (fatigued), suggesting a lack of compliance or understanding of the test protocol.
Figure 3 shows the mean percentage change (fatigue index) for each protocol in flexion and extension. Flexion protocols A, B, and D produced similar levels of fatigue, with protocol D producing the greatest fatigue: the mean peak torque after fatigue was 16.2% less than before, suggesting greatest sensitivity. Protocol C was the least effective at producing fatigue in flexion. Protocol D clearly produced the greatest fatigue in extension (8.8%).
With regard to a possible learning effect of the tasks over the four weeks, repeated measures analysis of variance revealed that there were differential effects depending on the type of task. The isokinetic value before fatigue for protocol B (week 3) was significantly (p<0.05) higher than that for protocol A (week 1) only in extension. For flexion, the isokinetic value before fatigue for protocol B (week 3) was significantly (p<0.05) lower than that for protocol A (week 1). For the isometric data, there were no differences between the peak values before fatigue at week 1 or 3 in either flexion or extension. These results suggest that learning was not a factor in this study.
Muscle fatigue is an important area of research and performance assessment, and consequently it has been the focus of many studies.4,6,24,25 It is a basic element of muscular performance that potentially has great relevance to activities of daily living, particularly in the trunk, where it is of importance for activities such as bending and lifting.3 Fatigue may also have an important role in athletic performance.26 However, the optimal method for assessing fatigue has not been clearly established. In fact, Mayer et al3 have suggested that in the back “the measurement of trunk muscle endurance remains a more elusive goal than strength measurement”.
What is already known on this topic
Fatigue has been highlighted to be of importance in the development of low back pain
In many studies on fatigue a clear definition has not been provided, and often tools used to measure fatigue have not been assessed or validated
One of the few validated measures of trunk fatigue is the Biering-Sorenson test,8,27 which is an isometric test of back extensor strength. However, its principle limitation in fatigue assessment is the fact that it is not a true measure of maximum voluntary contraction, with some suggesting that it records less than 50% of true maximum contraction3 and many questioning the reliability of the measures.3,28 There has also been criticism of this test, as it does not eliminate hip extensor activity.29 Also it is limited to the trunk extensors, and attempts to measure flexors with a view to recording fatigue ratios between the two muscle groups have been confounded by the use of submaximal tests performed either against or with the assistance of gravity.30
Other studies of trunk fatigue have examined the electromyographic activity of the back muscles using a protocol whereby an exercise, either the holding of an unsupported position such as in the Biering-Sorenson test8 or a series of timed flexion-extension movements, is performed until failure.31–33 However, there is little consensus on how many or what types of muscle tests are appropriate for inducing this failure. Arguments, however, exist as to what the electromyographic shifts observed are measuring,34 and inconsistencies have been noted between subjects with back pain and controls, the relevance of which is unclear as yet.32
The ability to study dynamic strength has existed for several years, with protocols primarily consisting of repeated contractions at 50% of maximal contraction until the point of fatigue. However, owing to the size and role of the trunk muscles, such methods were considered inappropriate by Mayer et al3 because they generated an unacceptably high anaerobic load. Thus, in that study, the subjects performed tests at maximal ability for fewer repetitions, and this was noted to be as reliable as isometric testing in a Roman Chair device.35 However, most studies have focused on isometric contractions.
Isometric tests of trunk fatigue and endurance have included chest raises,36,37 pulling tasks,38 and tests performed on sitting39–41 or standing dynamometers.23,42 During such tests, test position—that is, sitting or standing—has also been noted to affect the results, with Koumantakis et al43 suggesting that fatigue testing was more reliable in the upright position. Assessment of the impact of test position, however, was beyond the scope of this study.
Unfortunately, few studies have examined fatigue or endurance of the abdominal muscles, and few have compared isokinetic tasks with isometric tasks, although literature reviews tend to favour the use of isometric testing because of its low cost and equipment demands.29 Udermann et al44 examined the repeatability of endurance testing on a Roman Chair device and on a lumbar extension dynamometer, with endurance measured as time to maintain a set level of contraction or to hold a set position. Tests on the dynamometer included both static and dynamic tests, but the results of these different types of test were not compared, as in this study. However, their findings did suggest that all three methods had similar levels of repeatability. The emphasis of their study, however, was on endurance as opposed to fatigue and did not ascertain validity.
What this study adds
This initial study attempts to follow a published definition of fatigue, namely an “exercise induced reduction in maximal voluntary muscle force”
On the basis of this definition we have investigated different methods for generating and quantifying fatigue, in an attempt to provide a valid fatigue testing protocol
Our study was able to compare two different test protocols: isometric and isokinetic. Greater validity was shown in isometric testing. This can be seen in flexion, where protocol D produced the greatest percentage change in mean peak torque (16.2%). It was also the only method to induce significant fatigue in extension (8.8%). The finding that fatigue is more readily inducible in the abdominal muscle group than the back extensor muscle group is not new and corroborates findings of Smidt et al.45
There are several limitations to this work, the major one being the lack of randomisation of test procedures. However, closer inspection of the data does not show a learning effect. The other limitation is the limited test approaches as it may be that isokinetic testing is appropriate, but an insufficient number of repetitions were performed. This therefore warrants further investigation. Another potential area of concern is the ability of the isokinetic system to isolate muscle groups. It is acknowledged that such systems are unable to totally isolate muscle groups. Therefore further work may be required to investigate the contribution of the hip flexor and extensor groups, although during testing all attempts were made to minimise their activity.
The optimal method of measuring before and after fatigue is not clear. In flexion it can be seen that protocol A (isokinetic testing and fatigue) produces significant fatigue, but when the same isokinetic fatigue period was tested with isometric holds (protocol C), a significant level of fatigue was not produced. This may be the result of increased strength due to training in the previous protocols,1 but the timing of testing makes it unlikely that it is due to a learning curve in equipment use. This suggests that isokinetic testing is better at quantifying fatigue.
In extension, the opposite was true; protocol D (isometric testing and fatigue) produced the greatest fatigue, but when the same isometric fatigue period was tested isokinetically (protocol B), it failed to produce a significant level of fatigue. This suggests that isokinetic testing is inferior to isometric testing for determining fatigue. The reasons for this are unclear, although it could be postulated that a five second isometric hold is a more efficient way of achieving peak torque than a single isokinetic flexion and extension movement. Isometric testing was further proved to be superior in extension, revealing greater percentage change for the same fatiguing period when compared with isokinetic results. Isokinetic measurements also appeared less consistent: mean values before fatigue for protocols A and B (which should be similar) were significantly different (p<0.05) in both flexion and extension, whereas isometric mean measurements before fatigue were comparable in protocols C and D, especially in flexion where the values were 261.3 and 260.0 N.m respectively. It may be that a five second isometric hold is a more efficient way of achieving peak torque than a single isokinetic flexion and extension movement. Therefore isometric testing can be deemed more appropriate for testing fatigue than isokinetic methods. However, further work is needed to examine the repeatability of this test protocol.
During the study, on questioning, subjects perceived higher levels of exertion during protocols involving an isometric fatigue (B and D). It is therefore not surprising that these induced greater levels of fatigue. However, greater levels of perceived exertion do not necessarily correlate with greater levels of fatigue,24 although in this study there is support for this concept. A further important implicating factor is motivation, as all forms of endurance of fatigue testing depend on the motivation of the subject to complete the test to his or her own perceived limits of fatigue.29 We were unable to quantify or control for this factor, but we did attempt to minimise it by recruiting highly motivated and competitive athletes from the college rugby team.
Overall, the most effective method of inducing and measuring fatigue in the muscles of the trunk was protocol D—that is, isometric testing and fatigue. With mounting evidence that lack of trunk muscle endurance rather that actual strength is a predictor of low back pain, and that fatigue is potentially a key factor that may alter the loads on the spine and impose injury, it is important that a valid and repeatable method of testing fatigue is used in future research studies.
We thank the members of Imperial Medics RFC for their participation in this study and the ARC who funded the equipment for this study.
Competing interests: none declared
Written consent from the subject has been obtained for publication of figure 1 in print and online.
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