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001 AEROBIC EXERCISE TRAINING REDUCES PRE-BETA1 HDL GENERATION AND ENHANCES THE HDL3 ANTIOXIDANT ROLE IN TYPE 2 DIABETES MELLITUS SUBJECTS

I. C. D. Ribeiro1, R. T. Iborra1, M. Q. T. S. Neves1, S. A. Lottenberg1, A. M. Charf1, V. S. Nunes1, C. E. Negrão2, E. C. R. Quintão1, E. R. Nakandakare1, M. Passarelli1.1Lipids Laboratory (LIM-10), University of São Paulo Medical School, São Paulo, Brazil; 2Heart Institute, University of São Paulo Medical School, São Paulo, Brazil

Objectives: We analysed the influence of aerobic exercise training (AET) on plasma lipids, generation of pre-beta1 HDL, and HDL3 ability to remove cell cholesterol (chol) and inhibit LDL oxidation.

Methods: Healthy controls (C, n = 11) and type 2 diabetes mellitus patients (DMT, n = 11) were engaged in AET for 4 months, and compared to sedentary DM (DMS, n = 10). All subjects were submitted at admission and after 4 months to an oral fat load test with blood drawn at fasting (F) and 2, 4, 6 (PP), and 8 h after a fat-rich meal. HDL3 was isolated from F and PP plasmas and incubated with chol loaded cells to investigate chol efflux or with a healthy donor’s plasma LDL pool for measuring the lag time for LDL oxidation (LAG) on CuSO4 at 37°C (234 nm). Pre-beta1 HDL was measured by ELISA.

Results: V̇O2 maximal consumption was increased (10–20%) only in C and DMT. After AET, waist circumference was reduced, but there were no differences among the three groups regarding body weight, BMI, plasma total chol (TC), apoB, LDL-C, HDL-C, triglycerides (TG), glucose, insulin, HOMA-IR index, and areas under the curve of plasma TC, TG, and glucose. AET did not modify the F and PP composition of HDL3 or its ability to remove cell chol. In DMT alone, pre-beta1 HDL was higher …

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