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Chronic dynamic exercise increases apolipoprotein A-I expression in rabbit renal cortex as determined by proteomic technology
  1. R de Moraes1,
  2. R H Valente1,
  3. I R León1,
  4. M R O Trugilho1,
  5. A C L Nóbrega2,
  6. J Perales1,
  7. E Tibiriçá1
  1. 1
    Physiology and Pharmaco-dynamics Department, Oswaldo Cruz Institute, FIOCRUZ, Brazil
  2. 2
    Physiology and Pharmacology Department, Fluminense Federal University, Brazil
  1. E Tibiriçá, Laboratório de Farmacologia Neuro-Cardiovascular, Instituto Oswaldo Cruz, Fiocruz, Av. Brasil 4365 - Manguinhos, C P 926, 21045-900, Rio de Janeiro, RJ, Brazil; etibi{at}ioc.fiocruz.br

Abstract

Objective: We have shown previously that exercise training enhances endothelium-dependent and endothelium-independent vascular relaxation in rabbit kidney. This study aimed to investigate protein expression changes in the rabbit renal cortex induced by chronic dynamic exercise.

Design: Kidneys were obtained from New Zealand rabbits either confined to pens (n = 8) or trained on a treadmill (0% grade) for 5 days/week at a speed of 18 m/min for 60-min periods over 12 weeks (n = 8). Expression of proteins in the renal cortex was determined by colloidal Coomassie blue staining after two-dimensional polyacrylamide gel electrophoresis. Differential protein spots were excised and digested with trypsin, and peptides were sequenced by electrospray ionization-ion trap mass spectrometry.

Results: Two pairs of matching differentially stained spots displayed an approximate threefold increase in trained compared with sedentary animals. These four spots presented a molecular mass of 23 kDa but different pI values. Mass spectrometric analyses revealed the pairs of matching spots as being rabbit apolipoprotein A-I.

Conclusion: Chronic dynamic exercise increases apolipoprotein A-I expression in the rabbit renal cortex. This fact could be involved in the alterations observed in the renal circulation after exercise training.

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Footnotes

  • Competing interests: None.

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