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AUTOLOGOUS BLOOD DERIVED SIGNALING PROTEINS (SP) FOR TENDON AND MUSCLE THERAPY IN SPORTS MEDICINE. HIGHER CONCENTRATION OF GROWTH FACTORS (GF) AND CYTOKINES IN ORTHOKINE VS IN ACP
  1. MP Weisshaar,
  2. S Gaji
  1. Bonn-Rhein-Sieg University of Applied Sciences, Department of Natural Sciences, Von-Liebig-Straße 20, 53359 Rheinbach, Germany

Abstract

Regenerative SP in blood preparations are popular for tendon and muscle injuries [Wehling et al. Biodrugs 2007;21(5):323–332, Nguyen et al. PM&R 2011;(3):226–250, Schippinger et al. JSSM 2011;10:439–444]. They are used for local injection treatment. There is inconsistency in platelet-rich plasma (PRP) preparations (3x–7x platelets, variable other cells content). We compared 2 none-PRP techniques: ORTHOKINE (Oe) for Autologous Conditioned Serum (ACS) and ACP (Ax) for autologous plasma (AP). 9 Blood donors. Oe (10 mL) and Ax (12 mL). Blood processing: Oe: blood incubation in ORTHOKINE device 6 h @ 37°C, centrifugation to collect cell free serum. Ax: slow centrifugation of anticoagulated blood in ARTHREX ACP device to collect platelet containing plasma. Ax and whole blood cell counted. Samples stored at −20°C. ELISAs for SP: R&D Systems Kits. Results: Means: yield Ax: 3.4 mL; Oe: 3.7 mL. Ax: 85% platelet yield. GF: Oe over Ax 1.3x–3.9x. Cytokines: Oe over Ax: 2x–31x. Specifically: IL-1Ra: 5.5x; IL-10: 2x; IL-6: 31x; EGF: 3.9x; VEGF: 2.9x Oe over Ax. Discussion: Oe and Ax are used clinically. Degranulating platelets and other blood cells contribute to blood borne GF and cytokine output. Oe gains from all blood cells. Ax gains from 85% platelets. Oe's high IL-1Ra and GFs explain its profound (anti-inflammatory) action [Wehling et al. Biodrugs 2007;21(5):323–332]. Ax is not PRP, provides less SP than Oe. Muscle and tendon repair with Oe has been shown [Heisterbach et al. KSSTA 2012;10:1907–14, Majewski et al. Am J Sports Med 2009;11:2117–25, Wright-Carpenter et al. Int J Sports Med 2004;25:582–587 & 588–593]. Oe and Ax are used in joint treatment also. Oe has 2 y efficacy in high-pain OA [Baltzer et al. Osteoarthritis Cartilage 2009;17(2):152–160]. Autologous blood preparations are safe and, by WADA, not doping. ORTHOKINE® registered trademark of ORTHOGEN Inc. ARTHREX® ACP registered Trademark of ARTHREX Inc

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