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Br J Sports Med 47:i70-i73 doi:10.1136/bjsports-2013-092746
  • Original article

Ten days of simulated live high:train low altitude training increases Hbmass in elite water polo players

Open Access
  1. Christopher J Gore1,2,3
  1. 1National Institute of Sports Studies, University of Canberra, Canberra, Australia
  2. 2Department of Physiology, Australian Institute of Sport, Canberra, Australia
  3. 3Exercise Physiology Laboratory, Flinders University of South Australia, Adelaide, Australia
  1. Correspondence to Dr Laura A Garvican-Lewis, National Institute of Sports Studies, University of Canberra, Bruce, ACT 2601, Australia; laura.garvican{at}ausport.gov.au
  • Accepted 10 September 2013

Abstract

Objectives Water polo requires high aerobic power to meet the demands of match play. Live high:train low (LHTL) may enhance aerobic capacity at sea level. Before the Olympics, the Australian women's water polo team utilised LHTL in an attempt to enhance aerobic fitness.

Methods Over 6 months, 11 players completed three normobaric LHTL exposures (block 1:11 days at 3000 m; block 2+3:9 days at 2500 m, 11 days normoxia, 10 days at 2800 m). Haemoglobin mass (Hbmass) was measured through carbon monoxide-rebreathing. Before each block, the relationship between Hbmass and water polo-specific aerobic fitness was investigated using the Multistage Shuttle Swim Test (MSST). Effect size statistics were adopted with likely, highly likely and almost certainly results being >75%, >95%, >99%, respectively. A Pearson product moment correlation was used to characterise the association between pooled data of Hbmass and MSST.

Results Hbmass (mean±SD, pre 721±66 g) likely increased after block 1 and almost certainly after block 2+3 (% change; 90% confidence limits: block 1: 3.7%; 1.3–6.2%, block 2+3: 4.5%; 3.8–5.1%) and the net effect was almost certainly higher after block 2+3 than before block 1 (pre) by 8.5%; 7.3–9.7%. There was a very large correlation between Hbmass (g/kg) and MSST score (r=0.73).

Conclusions LHTL exposures of <2 weeks induced approximately 4% increase in Hbmass of water polo players. Extra Hbmass may increase aerobic power, but since match performance is nuanced by many factors it is impossible to ascertain whether the increased Hbmass contributed to Australia's Bronze medal.

Introduction

Water polo is a dynamic intermittent team sport, requiring a high aerobic power to meet the high-intensity demands of the game.1 ,2 When in the pool, field players are continuously involved in match play; requiring them to swim repeatedly from end to end, interspersed with high-intensity actions, including jumping, wrestling and sprinting.2 Indeed, the frequent occurrence of high-intensity activity bouts highlights the ability to perform such actions as an important facet of the game.2 Recovery from high-intensity actions requires the repletion of phosphocreatine which in turn, is reliant on aerobic metabolism. Therefore, a high aerobic power may also benefit repeat-sprint ability3 ,4 as well as improving overall match fitness.

Continuous swimming and small-sided games are routine training methods of elite water polo players; however, repetitive load on the shoulder carries increased injury risk.5 ,6 Therefore, novel interventions or activities which have potential to enhance aerobic capacity without increasing musculoskeletal load are attractive to team sports such as water polo, particularly in an Olympic year when player retention is critical.

Live high:train low (LHTL) altitude training is a popular training modality among endurance athletes and may enhance aerobic capacity at sea level7 through increase in haemoglobin mass (Hbmass).8 Although high-altitude training is not widely used by team sports as a means of enhancing intermittent high-intensity activity,9 a traditional live high:train High (LHTH) approach has been successfully utilised by some Australian rules football clubs in the preseason,10 showing promise as an effective intervention for enhancing aerobic fitness at sea level.

Before the 2012 Olympics, the Australian women's water polo team utilised simulated LHTL in an attempt to enhance aerobic fitness. Here, we document the changes in Hbmass during three LHTL exposures.

Methods

Subjects

The 2012 Olympic women's water polo training squad consisted of 20 players (4 goal keepers and 16 field players). Of these, altitude training was deemed as ‘high risk’ for five field players due to training age (junior athletes), illness or injury. The physical characteristics of the remaining 11 field players who engaged in altitude training were; age: 25.4±2.9 year, height: 178.0±6.6 cm and body mass: 77.8±10.8 kg. Athletes gave written informed consent before participating.

Altitude exposure

Over 6 months (October–March), a total of three simulated LHTL exposures were planned, comprising of 14 h/day at 2500–3000 m, in a five-bedroom normobaric hypoxic facility at the Australian Institute of Sport (AIS, Canberra, Australia) as follows: block 1 (n=9, October) comprised 11 days at 3000 m, (preseason); block 2 + 3 (n=11, February–March) comprised 9 days at 2500 m followed by 11 days normoxia (at home) and then 10 days at 2800 m, (6 weeks preinternational competition). During each altitude block, all training sessions, including water polo, swimming and weight training, were conducted in mild hypoxia (Canberra, 580 m). Venous blood was obtained at rest from an antecubital forearm vein prior to each altitude exposure to ascertain serum ferritin, with analysis performed on an Integra 400 (Roche Diagnostics, Switzerland) automated biochemistry analyser. All athletes were supplemented with oral iron daily throughout each camp (ferrous sulphate 305 mg). Athletes identified as iron deficient (serum ferritin <35 µg/L) prior to block 1 were instructed to continue supplementation between blocks 1 and 2, with serum ferritin levels assessed again prior to block 2+3.

Haemoglobin mass

Hbmass was measured using the optimised 2 min CO-rebreathing technique11 on five occasions: pre (before block 1), postblock 1, 2 weeks postblock 1, before block 2 and after block 3.

A CO bolus of 1.2 mL/kg body mass was rebreathed with 3 L of 100% oxygen for 2 min using a glass spirometer. Carboxyhaemoglobin (HbCO) concentration of capillary blood (finger-tip) was analysed using an OSM 3 hemoximeter (Radiometer, Copenhagen, Denmark) before and 7 min after inhalation of the bolus with the change in HbCO used to calculate Hbmass as described previously.12 ,13 The typical error for this test in our hands is 1.8%.14

Multistage shuttle swim test

At the start of blocks 1 and 2, aerobic fitness of all field players was assessed using the Multistage Shuttle Swim test (MSST).15 Players were required to continuously swim a series of 10 m shuttles between two lane ropes within a progressively decreasing cued time frame. The time allowed for each shuttle was reduced approximately every minute, with the swimming velocity required to meet each cue increased by 0.05 m/s. Players were removed from the test when they failed to be within one stroke of the lane rope on two consecutive occasions. The test has previously been validated with elite water polo players, with aerobic power shown to account for 78% of variance for MSST score.15 Further, the MSST is a suitable tool for discriminating players of different positions and playing standards16 as well as a valid method for obtaining maximum heart rate.17 Unfortunately, due to time and training constraints it was not possible to assess MSST following each hypoxic block. Therefore the effect of LHTL on aerobic fitness could not be determined.

Statistics

A contemporary approach to statistics was adopted since small changes in performance can be meaningful in athletes.18 Differences and associated 90% confidence limits (CL), in Hbmass changes after each altitude exposure were estimated to define the practical significance of the results, as opposed to a traditional significance and hypothesis testing. The magnitude of change within each block, as well as compared with premeasures was expressed as a standardised mean difference (Cohen effect sizes) computed as the difference in the mean divided by the between-subject SE, where a small effect is >0.2, moderate >0.6 and a large effect >1.2. The smallest worthwhile change was derived from Cohen's Scale for effect sizes in which a small effect is ≥0.2. Clear effects >75% likely positive were considered substantial, with the following descriptors attributed accordingly: 75–95%, likely; 95–99%, very likely; >99%, almost certainly.18

A Pearson product moment correlation was used to characterise the association between Hbmass and MSST. Data for every field player who conducted both the MSST and an Hbmass test at the same time point were pooled, regardless of whether the player subsequently engaged in the LHTL block. Pooled data comprised of 9 players prior to block 1 and 14 players prior to block 2. Effect sizes of correlation coefficients were defined as follows: trivial 0.0; small 0.1–0.3; moderate 0.3–0.5; large 0.5–0.7; very large 0.7–0.9; nearly perfect 0.9 and perfect 1.0.18

Data are expressed as the mean and SD unless otherwise stated. Data in graphs are presented as per cent changes from baseline measures.

Results

Prior to the first exposure mean (pre; mean±SD) Hbmass and ferritin were 721±66 g (9.4±0.7 g/kg) and 50.6±28.8 µg/L, respectively. A likely small increase in Hbmass (figure 1) was observed after block 1 (% change; 90% CL, effect size: block 1: 3.7%; 1.3–6.2%, 0.37) and remained 2 weeks later (4.0%; 1.9–6.1%, 0.40). Similarly, following block 2+3, an almost certainly small increase in Hbmass was observed (4.5%; 3.8–5.1%, 0.41) and was almost certainly moderately higher compared to pre-values (8.5%; 7.3–9.7%, 0.87; figure 2).

Figure 1

Individual (black circles) and mean changes (solid line) in haemoglobin mass (Hbmass) following each simulated live high: train low altitude exposure. Block 1: change in Hbmass following 11 days at 3000 m; 2 week post: changes in Hbmass versus premeasured 2 weeks post block 1; block 2+3: change in Hbmass following 9 days at 2500 m, followed by 11 days in normoxia then 10 days at 2800 m. Shaded area indicates typical error of Hbmass measurement.

Figure 2

Individual (black circles) and mean changes (solid line) in haemoglobin mass (Hbmass) following each simulated live high: train low altitude exposure compared to preseason values. Block 1:11 days at 3000 m; block 2+3: change in Hbmass following 9 days at 2500 m, followed by 11 days in normoxia then 10 days at 2800 m. Shaded area indicates typical error of Hbmass measurement.

Five players were identified at the start of block 1 as being iron deficient (ferritin <35 µg/L). At the start of block 2, mean ferritin had increased to 70.2±37.2 µg/L with only two players <35 µg/L.

A very large correlation was observed between Hbmass (g/kg) and MSST score (r=0.73; figure 3).

Figure 3

Relationship between haemoglobin mass (Hbmass) and the Multistage Shuttle Swim Test (MSST). Dotted lines indicate the 95% confidence band of regression line.

Discussion

The main finding of the present study was small but worthwhile changes in Hbmass of elite female water polo players were observed following simulated LHTL altitude exposures of 10–11 days.

Hbmass response

Previously, an increase in Hbmass of 1% per 100 h of exposure to either natural or simulated altitude has been documented19 ,20; leading to current recommendations for endurance athletes engaging in altitude training to aim for exposures of at least 2–3 weeks at greater than 2500 m.21 To our knowledge, the only other published data available showing Hbmass changes in team sport athletes after altitude training documents approximately 3% increase in Australian Rules football players after a 19-day training camp at 2130 m.10 The substantial erythropoietic response observed in these water polo players after a hypoxic dose of only 150–160 h is therefore surprising, but may be explained by the lower relative Hbmass of the players compared with elite endurance athletes.22 ,23 It should also be noted that the Hbmass time course and dose–response data available largely refers to endurance athletes and may not directly translate to athletes required to perform intermittent activity (eg, team sport). However, if the aerobic system of these ‘intermittent’ athletes is not maximally adapted through training,24 such athletes may present with a greater capacity for adaptation than previously expected. Further, the daily energy availability of team sport athletes may assist to create an optimal state of erythropoiesis25 compared with endurance athletes who may restrict energy intake.

The substantial and somewhat rapid Hbmass response observed may also be related to the timing of each block during the season. The players started block 1 in the base phase of training, 1 month after the annual training break or ‘off-season’. The exposure may have accelerated base phase training adaptations that usually occur over several weeks to months.26 ,27 Interestingly, large changes were observed after block 2+3, which was positioned in the precompetition phase. In the absence of altitude training, such changes mid-season would have required a substantial increase in training load—a 10% change in training load of elite cyclists has previously been related to only a 1% increase in Hbmass.14 Thus, in blocks 1 and 2+3, the use of simulated altitude training served to induce haematological adaptations that could potentially enhance oxygen transport capacity without requiring an increase in musculoskeletal load, and potentially decreasing injury risk.5 ,6 In a practical setting, the use of simulated LHTL may reduce the need for extensive swimming (or running for land-based sports), assisting in reducing the incidence of injury and induced fatigue, providing more time for skill-based and decision making drills.

A number of confounding situations have been identified which may blunt an erythropoietic response to altitude exposure—namely iron deficiency, illness, inflammation or insufficient energy availability.28 Five athletes were identified with serum ferritin <35 µg/L prior to the first exposure, and it is possible that their Hbmass response was blunted, despite the players being supplemented with oral iron. Indeed, the mean increase in Hbmass following block 2+3 was greater and coincided with a higher mean ferritin of the players at the start of the exposure, with only two players < 35µg/L.

Hbmass and MSST score

The relationship between Hbmass and VO2max has been described previously.29 The slope of the regression line suggests that at maximal performance, a 1 g increase in Hbmass is associated with an increase in VO2max of 4 mL/min. In the present study, MSST score was used as a surrogate for VO2max, displaying a strong correlation, and suggesting that increase in Hbmass may enhance aerobic power in these players. Unfortunately, since the MSST was not performed following each exposure we are unable to determine whether the observed changes in Hbmass did indeed result in improved water polo-specific fitness. What's more, whether altitude training induced adaptations, or indeed changes in fitness per se, translate to an improvement in team sport performance is near impossible to determine, due to the complexity of team sport play.30

At the 2012 Olympic games, the Australian team were required to play three games in succession which were extended into ‘extra-time,’ and ultimately won the bronze medal. Aerobic fitness was thus one of many important components in their preparation and performance.

Limitations

The present study documents a real world example of LHTL use by elite team sport athletes. However, the lack of a control group presents a limitation which must be acknowledged when interpreting our findings; since it is not known how much of the observed increase in Hbmass could have been achieved through training alone. The lack of a post-LHTL assessment of aerobic fitness is also a limitation of the study. Without such data we are unable to determine whether the LHTL exposures resulted in improved aerobic fitness in addition to the enhanced Hbmass observed, and thus scientific conclusions on the effectiveness of LHTL in this instance are difficult.

Conclusion

Relatively short duration exposures of simulated LHTL altitude training induced small increases in the Hbmass of elite women water polo players before the 2012 Olympics, which are likely to be beneficial to aerobic power and repeat sprint ability. However, since match performance is nuanced by many factors, including skill, tactics and refereeing decisions, it is impossible to ascertain whether the increased Hbmass contributed to Australia's bronze medal performance in London. Future research should investigate the relationship between altitude-induced changes in Hbmass and match fitness of team sport athletes.

What are the new findings?

  • Ten days of simulated live high:train low altitude training increased haemoglobin mass (Hbmass) of iron supplemented elite women water polo players by 3–4%.

  • The time course of the erythropoietic response to simulated altitude may be different for athletes engaged in high-intensity intermittent activity (such as team sport) as opposed to endurance athletes and may have implications for the optimal timing and hypoxic dose for team sports.

  • Hbmass of water polo players is strongly related to water polo-specific aerobic fitness.

How might the findings impact on clinical practice in the near future?

  • Simulated live high:train low may provide an alternative means of increasing oxygen transport capacity (through haemoglobin mass) in water polo players without inducing musculoskeletal load; however, whether these adaptations translate to improved match fitness or performance remains to be elucidated.

  • Iron status should be assessed in all athletes prior to engaging in altitude training.

  • Team sport athletes may not require the same hypoxic dose as endurance athletes.

Footnotes

  • Contributors LG, GM and TP were responsible for conception and design. LG and GM were involved in the acquisition of the data or analysis. LG, GM, SC, TP and CG were responsible for the interpretation of the data. LG, GM, SC, TP and CG were involved in drafting the article or revising it critically for important intellectual content. LG, GM, SC, TP and CG were responsible for the final approval of the version to be published.

  • Competing interests None.

  • Ethics approval Australian Institute of Sport Human Ethics Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/3.0/

References

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