Article Text

PDF
58 Smokers Have Worse Rotator Cuff Teartendon Degeneration And Apoptosis
  1. Kirsten Lundgreen1,
  2. Øystein Bjerkestrand Lian2,
  3. Alex Scott3,
  4. Angela Fearon3,
  5. Lars Engebretsen4
  1. 1Lovisenberg Diaconal Hospital, Norway
  2. 2Kristiansund Hospital HNR, Norway
  3. 3Department of Physical Therapy, University of British Columbia, Canada
  4. 4Oslo University Hospital, Norway

Abstract

Introduction Human studies indicate smokers to be more prone to rotator cuff tears and to present inferior results following rotator cuff repair compared to non-smokers [Baumgarten, 2010; Carbone, 2012; Kane, 2006; Kukkonen, 2012; Mallon, 2004]. We wished to assess the effect of smoking on the histopathology of supraspinatus tendinopathy.

Methods Supraspinatus tendon samples of 10 smokers and 15 non-smokers with full-thickness tears were harvested arthroscopically during rotator cuff repair. The severity of tendon histopathology including apoptosis, cellularity and proliferation was evaluated. Immunohistochemistry was used to assess the density of apoptotic cells (activated caspase-3; Asp175), proliferation (Ki67) and p53 (M7001)). Tendon degeneration was evaluated on tissue sections stained with H&E and Alcian blue according to a revised version of the modified Bonar score [Fearon, 2013].

Results The smokers were significantly younger (p = 0.01) and presented tears with a longer symptom duration than non-smokers (p < 0.05). The smokers’ tendons were characterised by more advanced degenerative changes (Bonar score, 13.5 [interquartile range, 1.4] v 9 [interquartile range, 3]; p < 0.001). These were accompanied by increased density of apoptotic cells (0.108 [SE, 0.038] v 0.0107 [SE, 0.007]; p = 0.024) (Figure 1), reduced tenocyte density (p = 0.019) and increased proliferative activity compared to torn supraspinatus tendons of non-smokers (p < 0.0001).

Abstract 58 Figure 1
Abstract 58 Figure 1

Apoptosis presented as amount of caspase 3- positive cells per square millimetre

Discussion Smoking is associated with early-onset tendon degeneration. Pronounced degenerative changes with reduced cell density and increased apoptotic cell death in smokers’ tendons may be associated with reduced tendon healing capacity. We recommend smoking to be controlled for in clinical studies on tendinopathy.

References Baumgarten KM et al. Clin Orthop Relat Res 2010;468(6):1534–1541

Carbone S et al. J Shoulder Elbow Surg 2012;21(1):56–60

Fearon A et al. J Sci Med Sport in press, online 8 August, 2013. doi:10.1016/j.jsams.2013.07.008

Kane SM et al. Orthopedics 2006;29(4):363–366

Kukkonen J et al. Scand J Med Sci Sports 2012;24:400–403

Mallon WJ et al. J Shoulder Elbow Surg 2004;13(2):129–132

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.