Different warm-up protocols (WUP) have been most commonly researched and it has been clearly demonstrated that some of these WUP elicit PAP however limited data are available about the best pre-conditioning activity (type of exercise, duration, volume, intensity). Therefore, our aim was to analyse 4 different WUP in order to examine the subsequent performance in countermovement jump (CMJ) and sprint performance at the same time, together with lactate along with performance in CMJ and sprint, what would be important and interesting information of the PAP-fatigue relationship for each one of protocols. Twelve high level sprinters (age 22 ± 2 y, body mass 70.2 ± 3.6 kg, height 181 ± 5 cm; body fat 7.4 ± 3.1%) took part in this study. Four different pre-conditioning activities (PCA) were designed, and after that, athletes randomly assigned to each one during 4-wk. PCA were squat (SQ) with 80% 1RM, (CMJ) with loads of 100% of body weight (BW), traditional WUP with three progressive sprint races and a specific sprint-based WUP composed by progressive sprint races. PAP effect was verified on CMJ and sprint performance in 0, 4, and 6 min after each WUP and group. CMJ height increased significantly at 0 and 4 min after all PCA protocols, showing a higher improvement in the specific sprint-based WUP (4.3%; p < 0.001; ES 0.93). Also a reduction in sprint time performance at 0 and 4 min was checked, with 2.6% (p < 0.01) with sprint-based WUP too. An interesting finding was that lactate values between 5.25 mmol·L^-1 and 7.74 mmol·L^-1 elicited PAP on sprint and CMJ performances for different WUP. Using a specific potentiation protocol that could enhance sprint performance would provide vital information, because running speed is critical in many athletic events. The knowledge of acute effects of PAP can be used to improve performance by including resistance exercises, jumps with extra load or sprint in an athlete’s warm-up (Mitchell and Sale, 2011, EJAP, 111: 1957–63).