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L-Carnitine antioxidant effect on protein concentration, acetylcholinesterase, (Na+,K+)-ATPase and Mg2+-ATPase activities in rat brain after forced swimming.
  1. Theodore Tsakiris
  1. Dept. of Experimental Physiology, Medical School, Athens University, Greece
    1. Panagoula Angelogianni
    1. Dept. of Experimental Physiology, Medical School, Athens University, Greece
      1. Christine Tesseromatis
      1. Dept. of Experimental Pharmacology, Medical School, Athens University, Greece
        1. Stylianos Tsakiris (stsakir{at}cc.uoa.gr)
        1. Dept. of Experimental Physiology, Medical School, Athens University, Greece
          1. Kleopatra H Schulpis
          1. Institute of Child Health, Aghia Sophia Childrens Hospital, Greece

            Abstract

            Background: Forced exercise produces free radicals and L-Carnitine (L-C) administration reduces oxidative stress. Aim: To investigate whether short (2h) or prolonged (3h) forced swimming could modulate total antioxidant status (TAS), protein concentration and activities of acetylcholinesterase (AChE), (Na+,K+)-ATPase and Mg2+-ATPase in rat brain following intraperitonal (ip) administration of L-C (300 mg/kg). Method: TAS, protein and enzyme activities were measured spectrophotometrically. Results: TAS, protein concentration and AChE activity were reduced, whereas Na+,K+-ATPase and Mg2+-ATPase were significantly increased post 2h or 3h of training. L-C administration resulted in a profound restoration of TAS and protein concentration whereas AChE and Na+,K+-ATPase were increased pre- exercise, followed by a AChE restoration and Na+,K+-ATPase reduction post exercise. Mg2+-ATPase remained unchanged. An in vitro study with L-C incubation of brain homogenates previously treated with L-C resulted in a complete restoration of the modulated enzymes, whereas the enzyme activities from untreated animals remained unaltered. Conclusions: Short or prolonged swimming in rats may result in a reduction of brain TAS, protein concentration and AChE activity, whereas Na+,K+-ATPase and Mg2+-ATPase were activated. L-C administration may restore TAS and protein concentration, whereas AChE and Na+,K+-ATPase activities were decreased, the latters reached pre-exercise values after L-C incubation.

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