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Sinusoidal analysis: a high resolution method for correlating biochemical reactions with physiological processes in activated skeletal muscles of rabbit, frog and crayfish

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Summary

A high resolution method for determining the complex stiffness of single muscle fibres is described. In this method the length of the fibre is oscillated sinusoidally, and the resulting force amplitude and phase shift are observed and interpreted in terms of chemo-mechanical energy transduction. In activated, fast skeletal muscles of rabbit (psoas), frog (semitendinosus) and crayfish (walking leg flexor), we resolved at least three exponential rate processes. We named these (A), (B), (C) in order of slow to fast. These processes should reflect ATP hydrolysis and concomitant energy transduction since they are absent in muscles that are relaxed, in rigor or fixed. The great similarities in the complex stiffness data from different muscles suggests that there is a common mechanism of chemo-mechanical energy transduction across a broad phylogenetic range.

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  1. Muscle Physiology Division, Departments of Neurology and Anatomy, Columbia University, 630 West 168th Street, 10032, New York, New York, USA

    Masataka Kawai & Philip W. Brandt

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  1. Masataka Kawai
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  2. Philip W. Brandt
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Kawai, M., Brandt, P.W. Sinusoidal analysis: a high resolution method for correlating biochemical reactions with physiological processes in activated skeletal muscles of rabbit, frog and crayfish. J Muscle Res Cell Motil 1, 279–303 (1980). https://doi.org/10.1007/BF00711932

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