Induction of IL-6 via the EP3 subtype of prostaglandin E receptor in rat adjuvant-arthritic synovial cells

Inflamm Res. 2001 Jan;50(1):1-5. doi: 10.1007/s000110050716.

Abstract

Objective and design: This study investigated the role of the prostaglandin E receptor EP3 subtype in the development of inflammatory synovitis.

Materials and methods: We examined the EP3 mRNA expression in the synovial tissue of rats with adjuvant arthritis and the effect of selective EP agonists on IL-6 production by cultured rat synovial cells.

Results: Of the two rat EP3 isoforms, rEP3B mRNA but not rEP3A mRNA was expressed in rat synovial cells. RT-PCR and ELISA were used to analyze the effects of selective EP3 agonist (ONO-AE-248) on IL-6 production by rat cultured synovial cells. This selective EP3 agonist induced IL-6 production by rat synovial cells in a time- and dose-dependent manner.

Conclusions: Our results suggest that PGE2 upregulates IL-6 production by synovial macrophages and fibroblasts through rEP3B in rats with adjuvant arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Experimental / metabolism*
  • Cells, Cultured
  • Dinoprostone / analogs & derivatives
  • Dinoprostone / pharmacology
  • Enzyme-Linked Immunosorbent Assay
  • Interleukin-6 / biosynthesis*
  • Male
  • RNA, Messenger / analysis
  • Rats
  • Rats, Inbred Lew
  • Receptors, Prostaglandin E / agonists
  • Receptors, Prostaglandin E / genetics
  • Receptors, Prostaglandin E / physiology*
  • Receptors, Prostaglandin E, EP3 Subtype
  • Reverse Transcriptase Polymerase Chain Reaction
  • Synovial Membrane / chemistry
  • Synovial Membrane / metabolism*

Substances

  • Interleukin-6
  • ONO AE 248
  • Ptger3 protein, rat
  • RNA, Messenger
  • Receptors, Prostaglandin E
  • Receptors, Prostaglandin E, EP3 Subtype
  • Dinoprostone