Laboratory variables and methods
| Substance | Medium | Method | Instrument |
|---|---|---|---|
| PAP, Peroxidase-antiperoxidase smear test; ELISA, enzyme linked immunosorbent assay. | |||
| Primary outcome variables | |||
| Triglyceride | Serum | Enzymatic (PAP) | Cobas Mira, Roche |
| Free fatty acids | Serum | Enzymatic | Cobas Mira, Roche |
| Secondary outcome variables | |||
| Glucose | Serum | Glucose dehydrogenase | Cobas Mira, Roche |
| Insulin | Serum | ELISA | ES (Enzym-System) 200, Boehringer |
| Lactate | Blood | Enzymatic | Cobas Mira, Roche |
| Haemoglobin | Blood | Photometric | K 1000, Digitana (Horgen) |
| Erythrocytes | Blood | Capillary count | K 1000, Digitana (Horgen) |