Abstract
This study examined the relationship between exercise-induced changes in the concentration of circulating immunocompetent cells and their surface expression of adhesion molecules: L-selectin (CD62L) and threeβ 2-integrins [LFA-1(CD11a/CD18), Mac-1 (CD11b/CD18), and p150/95(CD11c/CD18)]. Eight young male volunteers exercised on a cycle ergometer for 60 min at 60% maximal oxygen uptake. Peripheral blood samples, collected every 30 min throughout exercise and during the 2-h recovery period, were used for flow-cytometric analysis. The experimental results were compared with control data obtained ever 60 min at corresponding times of the nonexercise day. The exercise regimen induced a granulocytosis and a lymphocytosis, mainly due to an elevation of CD8+ and CD16+ cells. During recovery, a further granulocytosis occurred but accompanied by a lymphopenia. The increased CD8+ cell-count during exercise was characterized by a selective mobilization of the CD62L− and CD11ahigh cells, i.e.primed CD8+ cells. A postexercise suppression of CD4+ cell-count was derived only from CD62L+ cells. The CD11b+ and CD11c+ lymphocytes also increased during exercise, largely attributable to an increase in CD16+ cells which co-expressed CD11b and CD11c molecules. The CD62L surface density of granulocytes increased significantly during recovery. This resulted from a selective influx of CD62Lhigh granulocytes into the circulation. There were no significant changes in per-cell density of the threeβ 2-integrins on granulocytes and lymphocytes throughout the experimental period. These results suggest that the cell-surface expression of CD62L (and CD I la) molecules is associated with the differential mobilization of CD8+ cells during exercise, the postexercise suppression of CD4− cell-counts and the granulocytosis following exercise.
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References
Anderson DC, Miller LJ, Schmalstieg FC, Rothlein R, Springer TA (1986) Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies. J Immunol 137:15–27
Bevilacqua MP (1993) Endothelial-leukocyte adhesion molecules. Ann Rev Immunol 11:767–804
Bishop CR, Athens JW, Boggs DR, Warner HR, Cartwright GE, Wintrobe MM (1968) Leukokinetic studies. XIII. A non-steadystate kinetic evaluation of the mechanism of cortisone-induced granulocytosis. J Clin Invest 47:249–260
Boxer LA, Allen JM, Baehner RL (1980) Diminished polymorphonuclear leukocyte adherence-function dependent on release of cyclic AMP by endothelial cells after stimulation ofβ-recep-tors by epinephrine. J Clin Invest 66:268–274
Butcher EC (1991) Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67:1033–1036
Dustin ML, Springer TA (1989) T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature 341:619–624
Fauci AS (1975) Mechanisms of corticosteroid action on lymphocyte subpopulations. I. Redistribution of circulating T and B lymphocytes to the bone marrow. Immunology 28:669–680
Foster NK, Marlyn JB, Rangno RE, Hogg JC, Pardy RL (1986) Leukocytosis of exercise: role of cardiac output and catecholamines. J Appl Physiol 61:2218–2223
Gearing AJH, Niwman W (1993) Circulating adhesion molecules in disease. Immunol Today 14:506–512
Hansen JB, Wilsgard L, Osterud B (1991) Biphasic changes in leukocytes induced by strenuous exercise. Eur J Appl Physiol 62:157–161
Hynes RD (1992) Integrins: versality, modulation, and signaling in cell adhesion. Cell 69:11–25
Iversen PO, Stokland A, Rolstad B, Benestad HB (1994) Adrenalineinduced leucocytosis: recruitment of blood cells from rat spleen, bone marrow and lymphatics. Eur J Appl Physiol 68:219–227
Keast D, Cameron K, Morton AR (1988) Exercise and the immune response. Sports Med 5:248–267
Kishimoto TK, Jutila MA, Berg EL, Butcher EC (1989) Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science 245:1228–1241
Lawrence MB, Springer TA (1991) Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 65:859–873
Lo SK, Detmers PA, Levin SM, Wright SD (1989) Transient adhesion of neutrophils to endothelium. J Exp Med 169:1779–1793
Mackay CR (1991) T-cell memory. The connection between function, phenotype and migration pathways. Immunol Today 12:189–192
Mackay CR, Imhof BA (1993) Cell adhesion in the immune system. Immunol Today 14:99–102
Nieman DC, Miller AR, Henson DA, Warren BJ, Gusewitch G, Johnson RL, Davis JM, Butterworth DE, Herring JL, Nehlsen-Cannarella SL (1994) Effect of high- versus moderate-intensity exercise on lymphocyte subpopulations and proliferative response. Int J Sports Med 15:199–206
Ohashi Y, Takeshita T, Nagata K, Mori S, Sugamura K (1989) Differential expression of the IL-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells. J Immunol 143:3548–3555
Okumura M, Fujii Y, Takeuchi Y, Inada K, Nakahara K, Matsuda H (1993) Age-related accumulation of LFA-1high cells in a CD8+CD45RAhigh T cell population. Eur J Immunol 23:1057–1063
Picker LJ, Butcher EC (1992) Physiological and molecular mechanisms of lymphocyte homing. Ann Rev Immunol 10:561–591
Picker LJ, Terstappen LW, Rott LS, Streeter PR, Stein H, Butcher EC (1990) Differential expression of homing-associated adhesion molecules by T cell subsets in man. J Immunol 145: 3247–3255
Rothlein R, Springer TA (1986) The requirement of lymphocyte function-associated antigen 1 in homotypic leukocyte adhesion stimulated by phorbol ester. J Exp Med 163:1132–1149
Sanders ME, Makgoba MW, Sharrow SO, Stephany D, Springer TA, Young HA, Shaw S (1988) Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UHLI, CDw29, and Pgp-1) and have enhanced IFN-gamma production. J Immunol 140:1401–1407
Shimizu Y, Newman W, Tanaka Y, Shaw S (1992) Lymphocyte interactions with endothelial cells. Immunol Today 13:106–112
Shinkai S, Shore S, Shek PN, Shephard RJ (1992) Acute exercise and immune function. Relationship between lymphocyte activity and changes in subset counts. Int J Sports Med 13:452–461
Simon HB (1984) The immunology of exercise: a brief review. J Am Med Assoc 252:2735–2738
Spertini O, Kansas GS, Munro JM, Griffin JD, Tedder TF (1991) Regulation of leukocyte migration by activation of the leukocyte adhesion molecule-1 (LAM-1) selectin. Nature 349:691–694
Stoolman LM (1993) Adhesion molecules involved in leukocyte recruitment and lymphocyte recirculation. Chest 103:79S-86S
Takeuchi M (1991) Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251:1451–1455
Tedder TF, Matsuyama T, Rothstein D, Schlossman SF, Morimoto C (1990) Human antigen-specific memory T cells express the homing receptor(LAM-1) necessary for lymphocyte recirculation. Eur J Immunol 20:1351–1355
Tvede N, Kappel M, Klarlund K, Duhn S, Jalkjaer-Kristensen J, Kjaer M, Galbo H, Pedersen BK (1994) Evidence that the effect of bicycle exercise on blood mononuclear cell proliferative responses and subsets is mediated by epinephrine. Int J Sports Med 15:100–104
Williams AF, Barclay AN (1988) The immunoglobulin superfamily: domains for cell surface recognition. Annu Rev Immunol 6:381–405
Zimmerman GA, Prescott SM, McIntyre TM (1992) Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today 13:93–100
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Kurokawa, Y., Shinkai, S., Torii, J. et al. Exercise-induced changes in the expression of surface adhesion molecules on circulating granulocytes and lymphocytes subpopulations. Europ. J. Appl. Physiol. 71, 245–252 (1995). https://doi.org/10.1007/BF00854986
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DOI: https://doi.org/10.1007/BF00854986