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Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

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Abstract

To investigate the acute effect of a hot, humid and ozone-polluted (O3) environment on lung inflammation and oxidative tress of runners performing 8 km time trial run. Using a single-blinded randomized design, 10 male athletes (mean\( \dot{V}{\text{O}}_{2\max } \)= 64.4 mlO2 kg−1 min−1, SD = 4.4) took part in a time trial run in four different environmental conditions: 20°C + 50% relative humidity (rh) (Control); 20°C + 50% rh + 0.10 ppm O3 (Control + O3); 31°C + 70% rh (Heat); 31°C + 70% rh + 0.10 ppm O3 (Heat + O3). Blood samples and nasal lavage were collected post-exercise and analyzed for inflammatory, epithelial damage and oxidative stress markers. Data were analyzed using repeated measures ANOVA with Tukey’s post hoc test. A significant increase in CC16 concentration (P < 0.05) and GSH/protein concentration (P < 0.05) in the upper respiratory airways was observed following the 8 km run in the Heat + O3 trial compared with the control trial. There were no differences in the neutrophil counts between trials. No differences were observed for the other antioxidants analyzed. A hot, humid and ozone-polluted environment (0.1 ppm) elicits an early epithelial damage and antioxidant protection process in the upper respiratory airways of athletes immediately after performing 8 km time trial run.

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Acknowledgments

This study was supported by Edinburgh Napier University research grant.

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Authors and Affiliations

  1. Biomedicine and Sports Science Research Group, Edinburgh Napier University, Merchiston Campus, 10 Colinton Road, Edinburgh, EH10 5DT, UK

    Elisa Couto Gomes, Vicki Stone & Geraint Florida-James

Authors
  1. Elisa Couto Gomes
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  2. Vicki Stone
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  3. Geraint Florida-James
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Correspondence to Elisa Couto Gomes.

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Communicated by George Havenith.

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Gomes, E.C., Stone, V. & Florida-James, G. Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run. Eur J Appl Physiol 111, 2089–2097 (2011). https://doi.org/10.1007/s00421-011-1839-x

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  • DOI: https://doi.org/10.1007/s00421-011-1839-x

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