Elsevier

Clinical Biochemistry

Volume 29, Issue 4, August 1996, Pages 301-308
Clinical Biochemistry

Use of enzyme immunoassay for measurement of skeletal troponin-I utilizing isoform-specific monoclonal antibodies

https://doi.org/10.1016/0009-9120(96)00016-1Get rights and content

Abstract

Objectives: To determine the serum level of fast skeletal troponin I (fsTnI) resulting from skeletal muscle damage, we have developed a sensitive two-site enzyme immunoassay to measure skeletal troponin I.

Design and Methods: Twelve monoclonal antibodies were raised against human fsTnI. Of these antibodies, 8 were fsTnI-specific and the remaining 4 reacted with both skeletal and cardiac troponin I (cTnI). Two monoclonals were utilized for a development of this fsTnI immunoassay. Standards were made with purified recombinant human fsTnI for the range of 0–25 μg/mL.

Results: Total assay variance (CV) ranged from 1.7% to 9.6%. The upper limit of the normal reference range was established as 0.2 μg/L by determining fsTnI concentration in sera of 108 healthy donors without evidence of muscle damage. Purified human cTnI up to 500 μg/L and cTnI-positive clinical serum samples yielded negative results in the fsTnI assay. The serum levels of fsTnI were determined in trauma patients, patients with chronic degenerative muscle disease, and marathon runners. In the study populations, the serum levels of fsTnI were correlated with other biochemical markers that are traditionally used to monitor striated muscle damage.

Conclusions: In the present preliminary studies, measuring the serum levels of fsTnI in patients with various forms of muscle damage is more accurate than using the classical non muscle-specific biochemical markers.

References (21)

There are more references available in the full text version of this article.

Cited by (28)

  • The Troponin-I fast skeletal muscle is reliable marker for the determination of vitality in the suicide hanging

    2019, Forensic Science International
    Citation Excerpt :

    The commonly used serum markers, e.g., lactate dehydrogenase, creatine kinase (CK), 1 and myoglobin, lack specificity to skeletal tissue. Several groups [11–14] used immunoassays to investigate use of the skeletal isoform of troponin I (sTnI) as a marker of skeletal muscle injury, just as its cardiac isoform (cTnI) is currently the gold standard for detecting cardiac muscle injury [15]. sTnI exists as 2 different isoforms, slow sTnI (ssTnI) and fast sTnI (fsTnI), produced in slow- (ST) and fast-twitch (FT) fibers, respectively.

  • Biomarker panel of cardiac and skeletal muscle troponins, fatty acid binding protein 3 and myosin light chain 3 for the accurate diagnosis of cardiotoxicity and musculoskeletal toxicity in rats

    2012, Toxicology
    Citation Excerpt :

    Therefore, while increases in these conventional biomarkers make it possible to detect a broad spectrum of organ toxicity, these biomarkers cannot discriminate between specific organ toxicities. In order to complement the low tissue specificity, several promising biomarkers such as cardiac and skeletal muscle troponins (cTnI, cTnT and sTnI), fatty acid binding protein-3 (FABP3) and myosin light chain-3 (MYL3, also known as MLC1V) have also been adapted for clinically monitoring patients (Colli et al., 2007; Keller et al., 2009; Hillis et al., 2003; Matziolis et al., 2011; Pelsers et al., 2005; Ravkilde et al., 1995; Reichlin et al., 2009; Simpson et al., 2005; Takahashi et al., 1996). Therefore, the reverse-translational application of these promising biomarkers in experimental animals could potentially improve the detection power for diagnosing cardiotoxicity and musculoskeletal toxicity, in addition to helping predict the occurrence of these toxicities in humans.

  • Electrochemiluminescent immunoassay for rat skeletal troponin I (Tnni2) in serum

    2010, Journal of Pharmacological and Toxicological Methods
  • Biomarkers in glycogen storage diseases: An update

    2021, International Journal of Molecular Sciences
View all citing articles on Scopus
View full text