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Guidelines for Glycerol Use in Hyperhydration and Rehydration Associated with Exercise

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Abstract

Dehydration in athletes alters cardiovascular and thermoregulatory function and may inhibit endurance exercise capacity if fluid loss exceeds 2% of bodyweight (BW). If this level of dehydration cannot be prevented when starting from a state of euhydration, then athletes may create a state of hyperhydration by consuming extra fluid prior to exercise. From this hyperhydrated situation, individuals have a greater capacity to tolerate fluid loss before becoming dehydrated. Furthermore, excess pre-exercise fluid intake enhances thermoregulatory ability, as well as increasing plasma volume to maintain cardiac output. However, hyperhydrating before exercise is difficult, because a large fluid intake is typically accompanied by diuresis. Glycerol-containing beverages create an osmotic gradient in the circulation favouring fluid retention, thereby facilitating hyperhydration and protecting against dehydration. Many studies have shown that increases in body water by 1L or more are achievable through glycerol hyperhydration. This article analyses the evidence for glycerol use in facilitating hyperhydration and rehydration, and provides guidelines for athletes wishing to use this compound. An analysis of the studies in this area indicates that endurance athletes intending to hyperhydrate with glycerol should ingest glycerol 1.2 g/kg BW in 26 mL/kg BW of fluid over a period of 60 minutes, 30 minutes prior to exercise. The effects of glycerol on total body water when used during rehydration are less well defined, due to the limited studies conducted. However, ingesting glycerol 0.125 g/kg BW in a volume equal to 5mL/kg BW during exercise will delay dehydration, while adding glycerol 1.0 g/kg BW to each 1.5L of fluid consumed following exercise will accelerate the restoration of plasma volume. Side effects from glycerol ingestion are rare, but include nausea, gastrointestinal discomfort and light-headedness. In summary, glycerol ingestion before, during or following exercise is likely to improve the hydration state of the endurance athlete.

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References

  1. Riedesel ML, Allen DY, Peake GT, et al. Hyperhydration with glycerol solutions. J Appl Physiol 1987 Dec; 63 (6): 2262–8

    PubMed  CAS  Google Scholar 

  2. Gonzalez-Alonso J, Mora-Rodriguez R, Below PR, et al. Dehydration reduces cardiac output and increases systemic and cutaneous vascular resistance during exercise. J Appl Physiol 1995 Nov; 79 (5): 1487–96

    PubMed  CAS  Google Scholar 

  3. Nadel ER, Fortney SM, Wenger CB. Effect of hydration state of circulatory and thermal regulations. J Appl Physiol 1980 Oct; 49 (4): 715–21

    PubMed  CAS  Google Scholar 

  4. Sawka MN, Young AJ, Francesconi RP, et al. Thermoregulatory and blood responses during exercise at graded hypohydration levels. J Appl Physiol 1985 Nov; 59 (5): 1394–401

    PubMed  CAS  Google Scholar 

  5. Gonzalez-Alonso J, Calbet JA, Nielsen B. Muscle blood flow is reduced with dehydration during prolonged exercise in humans. J Physiol 1998 Dec 15; 513 (Pt3): 895–905

    Article  PubMed  CAS  Google Scholar 

  6. Gonzalez-Alonso J, Calbet JA. Reductions in systemic and skeletal muscle blood flow and oxygen delivery limit maximal aerobic capacity in humans. Circulation 2003 Feb 18; 107 (6): 824–30

    Article  PubMed  Google Scholar 

  7. Hubbard RW. The role of exercise in the etiology of exertional heatstroke. Med Sci Sports Exerc 1990 Feb; 22 (1): 2–5

    PubMed  CAS  Google Scholar 

  8. Coris EE, Ramirez AM, Van Durme DJ. Heat illness in athletes: the dangerous combination of heat, humidity and exercise. Sports Med 2004; 34 (1): 9–16

    Article  PubMed  Google Scholar 

  9. Armstrong LE, Casa DJ, Millard-Stafford M, et al. American College of Sports Medicine position stand: exertional heat illness during training and competition. Med Sci Sports Exerc 2007 Mar; 39 (3): 556–72

    Article  PubMed  Google Scholar 

  10. Walsh RM, Noakes TD, Hawley JA, et al. Impaired highintensity cycling performance time at low levels of dehydration. Int J Sports Med 1994 Oct; 15 (7): 392–8

    Article  PubMed  CAS  Google Scholar 

  11. Below PR, Mora-Rodriguez R, Gonzalez-Alonso J, et al. Fluid and carbohydrate ingestion independently improve performance during 1 h of intense exercise. Med Sci Sports Exerc 1995 Feb; 27 (2): 200–10

    PubMed  CAS  Google Scholar 

  12. Armstrong LE, Costill DL, Fink WJ. Influence of diureticinduced dehydration on competitive running performance. Med Sci Sports Exerc 1985 Aug; 17 (4): 456–61

    Article  PubMed  CAS  Google Scholar 

  13. Cheuvront SN, Carter 3rd R, Castellani JW, et al. Hypohydration impairs endurance exercise performance in temperate but not cold air. J Appl Physiol 2005 Nov; 99 (5): 1972–6

    Article  PubMed  Google Scholar 

  14. Craig EN, Cummings EG. Dehydration and muscular work. J Appl Physiol 1966 Mar; 21 (2): 670–4

    PubMed  CAS  Google Scholar 

  15. Pichan G, Gauttam RK, Tomar OS, et al. Effect of primary hypohydration on physical work capacity. Int J Biometeorol 1988 Sep; 32 (3): 176–80

    Article  PubMed  CAS  Google Scholar 

  16. Nybo L, Jensen T, Nielsen B, et al. Effects of marked hyperthermia with and without dehydration on VO(2) kinetics during intense exercise. J Appl Physiol 2001 Mar; 90 (3): 1057–64

    PubMed  CAS  Google Scholar 

  17. Wingo JE, Casa DJ, Berger EM, et al. Influence of a preexercise glycerol hydration beverage on performance and physiologic function during mountain-bike races in the heat. J Athl Train 2004 Jun; 39 (2): 169–75

    PubMed  Google Scholar 

  18. Sawka MN, Burke LM, Eichner ER, et al. American College of Sports Medicine position stand: exercise and fluid replacement. Med Sci Sports Exerc 2007 Feb; 39 (2): 377–90

    Article  PubMed  Google Scholar 

  19. Wagner DR. Hyperhydrating with glycerol: implications for athletic performance. J Am Diet Assoc 1999 Feb; 99 (2): 207–12

    Article  PubMed  CAS  Google Scholar 

  20. Lyons TP, Riedesel ML, Meuli LE, et al. Effects of glycerolinduced hyperhydration prior to exercise in the heat on sweating and core temperature. Med Sci Sports Exerc 1990 Aug; 22 (4): 477–83

    PubMed  CAS  Google Scholar 

  21. Kavouras SA, Armstrong LE, Maresh CM, et al. Rehydration with glycerol: endocrine, cardiovascular and thermoregulatory responses during exercise in the heat. J Appl Physiol 2006; 100 (2): 442–50

    Article  PubMed  CAS  Google Scholar 

  22. Figaro MK, Mack GW. Regulation of fluid intake in dehydrated humans: role of oropharyngeal stimulation. Am J Physiol 1997 Jun; 272 (6Pt2): R1740–6

    PubMed  CAS  Google Scholar 

  23. Robergs RA, Griffin SE. Glycerol: biochemistry, pharmacokinetics and clinical and practical applications. Sports Med 1998 Sep; 26 (3): 145–67

    Article  PubMed  CAS  Google Scholar 

  24. Nelson JL, Robergs RA. Exploring the potential ergogenic effects of glycerol hyperhydration. Sports Med 2007; 37 (11): 981–1000

    Article  PubMed  Google Scholar 

  25. Goulet ED, Aubertin-Leheudre M, Plante GE, et al. Ametaanalysis of the effects of glycerol-induced hyperhydration on fluid retention and endurance performance. Int J Sport Nutr Exerc Metab 2007 Aug; 17 (4): 391–410

    PubMed  CAS  Google Scholar 

  26. Freund BJ, Montain SJ, Young AJ, et al. Glycerol hyperhydration: hormonal, renal, and vascular fluid responses. J Appl Physiol 1995 Dec; 79 (6): 2069–77

    PubMed  CAS  Google Scholar 

  27. Melin B, Jimenez C, Koulmann N, et al. Hyperhydration induced by glycerol ingestion: hormonal and renal responses. Can J Physiol Pharmacol 2002 Jun; 80 (6): 526–32

    Article  PubMed  CAS  Google Scholar 

  28. Koulmann N, Jimenez C, Regal D, et al. Use of bioelectrical impedance analysis to estimate body fluid compartments after acute variations of the body hydration level. Med Sci Sports Exerc 2000 Apr; 32 (4): 857–64

    Article  PubMed  CAS  Google Scholar 

  29. Meyer LG, Horrigan VJr DJ, Lotz WG. Effects of three hydration beverages on exercise performance during 60 hours of heat exposure. Aviat Space Environ Med 1995 Nov; 66 (11): 1052–7

    PubMed  CAS  Google Scholar 

  30. Montner P, Stark DM, Riedesel ML, et al. Pre-exercise glycerol hydration improves cycling endurance time. Int J Sports Med 1996 Jan; 17 (1): 27–33

    Article  PubMed  CAS  Google Scholar 

  31. Latzka WA, Sawka MN, Montain SJ, et al. Hyperhydration: thermoregulatory effects during compensable exercise-heat stress. J Appl Physiol 1997 Sep; 83 (3): 860–6

    PubMed  CAS  Google Scholar 

  32. Latzka WA, Sawka MN, Montain SJ, et al. Hyperhydration: tolerance and cardiovascular effects during uncompensable exercise-heat stress. J Appl Physiol 1998 Jun; 84 (6): 1858–64

    PubMed  CAS  Google Scholar 

  33. Hitchins S, Martin DT, Burke L, et al. Glycerol hyperhydration improves cycle time trial performance in hot humid conditions. Eur J Appl Physiol Occup Physiol 1999 Oct; 80 (5): 494–501

    Article  PubMed  CAS  Google Scholar 

  34. Montner P, Zou Y, Robergs R, et al. Glycerol hyperhydration alters cardiovascular and renal function. J Exerc Physiol (Online) 1999; 2 (1): 1–10

    Google Scholar 

  35. Anderson MJ, Cotter JD, Garnham AP, et al. Effect of glycerol-induced hyperhydration on thermoregulation and metabolism during exercise in heat. Int J Sport Nutr Exerc Metab 2001 Sep; 11 (3): 315–33

    Article  PubMed  CAS  Google Scholar 

  36. Coutts A, Reaburn P, Mummery K, et al. The effect of glycerol hyperhydration on Olympic distance triathlon performance in high ambient temperatures. Int J Sport Nutr Exerc Metab 2002 Mar; 12 (1): 105–19

    PubMed  CAS  Google Scholar 

  37. Magal M, Webster MJ, Sistrunk LE, et al. Comparison of glycerol and water hydration regimens on tennis-related performance. Med Sci Sports Exerc 2003 Jan; 35 (1): 150–6

    Article  PubMed  CAS  Google Scholar 

  38. Marino FE, Kay D, Cannon J. Glycerol hyperhydration fails to improve endurance performance and thermoregulation in humans in a warm humid environment. Pflugers Arch 2003 Jul; 446 (4): 455–62

    Article  PubMed  CAS  Google Scholar 

  39. Goulet ED, Robergs RA, Labrecque S, et al. Effect of glycerol-induced hyperhydration on thermoregulatory and cardiovascular functions and endurance performance during prolonged cycling in a 25 degrees C environment. Appl Physiol Nutr Metab 2006 Apr; 31 (2): 101–9

    Article  PubMed  Google Scholar 

  40. Easton C, Turner S, Pitsiladis YP. Creatine and glycerol hyperhydration in trained subjects before exercise in the heat. Int J Sport Nutr Exerc Metab 2007 Feb; 17 (1): 70–91

    PubMed  CAS  Google Scholar 

  41. Nishijima T, Tashiro H, Kato M, et al. Alleviation of exercise-induced dehydration under hot conditions by glycerol hyperhydration. Int J Sport Health Sci 2007; 5: 32–41

    Article  Google Scholar 

  42. Dini M, Corbianco S, Rossi B, et al. Hyperhydrating with glycerol: effects on thermoregulation, hydration and athletic performance during specific exergonic exercise in a warm-humid environment. Sport Sci Health 2007; 2: 1–7

    Article  Google Scholar 

  43. Goulet ED, Rousseau SF, Lamboley CR, et al. Pre-exercise hyperhydration delays dehydration and improves endurance capacity during 2 h of cycling in a temperate climate. J Physiol Anthropol 2008 Sep; 27 (5): 263–71

    Article  PubMed  Google Scholar 

  44. Murray R, Eddy DE, Paul GL, et al. Physiological responses to glycerol ingestion during exercise. J Appl Physiol 1991 Jul; 71 (1): 144–9

    PubMed  CAS  Google Scholar 

  45. Siegler JC, Mermier CM, Amorim FT, et al. Hydration, thermoregulation, and performance effects of two sport drinks during soccer training sessions. J Strength Cond Res 2008 Sep; 22 (5): 1394–401

    Article  PubMed  Google Scholar 

  46. Scheett TP, Webster MJ, Wagoner KD. Effectiveness of glycerol as a rehydrating agent. Int J Sport Nutr Exerc Metab 2001 Mar; 11 (1): 63–71

    PubMed  CAS  Google Scholar 

  47. Koenigsberg PS, Martin KK, Hlava HR, et al. Sustained hyperhydration with glycerol ingestion. Life Sci 1995; 57 (7): 645–53

    Article  PubMed  CAS  Google Scholar 

  48. Jadad AR, Moore RA, Carroll D, et al. Assessing the quality of reports of randomized clinical trials: is blinding necessary? Control Clin Trial 1996 Feb; 17 (1): 1–12

    Article  CAS  Google Scholar 

  49. Maher CG, Moseley AM, Sherrington C, et al. A description of the trials, reviews, and practice guidelines indexed in the PE Dro database. Phys Ther 2008 Sep; 88 (9): 1068–77

    Article  PubMed  Google Scholar 

  50. Verhagen AP, de Vet HC, de Bie RA, et al. The Delphi list: a criteria list for quality assessment of randomized clinical trials for conducting systematic reviews developed by Delphi consensus. J Clin Epidemiol 1998 Dec; 51 (12): 1235–41

    Article  PubMed  CAS  Google Scholar 

  51. Moher D, Schulz KF, Altman DG. The CONSORT statement: revised recommendations for improving the quality of reports of parallel-group randomised trials. Lancet 2001 Apr 14; 357 (9263): 1191–4

    Article  PubMed  CAS  Google Scholar 

  52. Moher D, Pham B, Jones A, et al. Does quality of reports of randomised trials affect estimates of intervention efficacy reported in meta-analyses? Lancet 1998 Aug 22; 352 (9128): 609–13

    Article  PubMed  CAS  Google Scholar 

  53. Pengel LH, Barcena L, Morris PJ. The quality of reporting of randomized controlled trials in solid organ transplantation. Transpl Int 2009; 22 (4): 377–84

    Article  PubMed  Google Scholar 

  54. Inder WJ, Swanney MP, Donald RA, et al. The effect of glycerol and desmopressin on exercise performance and hydration in triathletes. Med Sci Sports Exerc 1998 Aug; 30 (8): 1263–9

    Article  PubMed  CAS  Google Scholar 

  55. Coombes JS, Hamilton KL. The effectiveness of commercially available sports drinks. Sports Med 2000 Mar; 29 (3): 181–209

    Article  PubMed  CAS  Google Scholar 

  56. Von Duvillard SP, Braun WA, Markofski M, et al. Fluids and hydration in prolonged endurance performance. Nutrition 2004 Jul-Aug; 20 (7-8): 651–6

    Article  Google Scholar 

  57. Allen LA, Teichberg S, et al. Proabsorptive effect of glycerol as a glucose substitute in oral rehydration solutions. J Nutr Biochem 1999 Jan; 10 (1): 49–55

    Article  PubMed  CAS  Google Scholar 

  58. Wapnir RA, Sia MC, Fisher SE. Enhancement of intestinal water absorption and sodium transport by glycerol in rats. J Appl Physiol 1996 Dec; 81 (6): 2523–7

    PubMed  CAS  Google Scholar 

  59. Lamb DR, Lightfoot WS, Myhal M. Prehydration with glycerol does not improve cycling performance vs 6% CHO-electrolyte drink [abstract]. Med Sci Sports Exerc 1997; 29 (5 Suppl.): 249

    Google Scholar 

  60. Speedy DB, Faris JG, Hamlin M, et al. Hyponatremia and weight changes in an ultradistance triathlon. Clin J Sport Med 1997 Jul; 7 (3): 180–4

    Article  PubMed  CAS  Google Scholar 

  61. Speedy DB, Noakes TD, Rogers IR, et al. Hyponatremia in ultradistance triathletes. Med Sci Sports Exerc 1999 Jun; 31 (6): 809–15

    Article  PubMed  CAS  Google Scholar 

  62. Noakes TD, Sharwood K, Speedy D, et al. Three independent biological mechanisms cause exercise-associated hyponatremia: evidence from 2,135 weighed competitive athletic performances. Proc Nat Acad Sci USA 2005 Dec 20; 102 (51): 18550–5

    Article  PubMed  CAS  Google Scholar 

  63. Noakes T. Hyponatremia in distance runners: fluid and sodium balance during exercise. Curr Sports MedRep 2002 Aug; 1 (4): 197–207

    PubMed  Google Scholar 

  64. Irving RA, Noakes TD, Buck R, et al. Evaluation of renal function and fluid homeostasis during recovery from exerciseinduced hyponatremia. J Appl Physiol 1991 Jan; 70 (1): 342–8

    PubMed  CAS  Google Scholar 

  65. Murray B, Eichner ER. Hyponatremia of exercise. Curr Sports Med Rep 2004 Jun; 3 (3): 117–8

    PubMed  Google Scholar 

  66. Vrijens DM, Rehrer NJ. Sodium-free fluid ingestion decreases plasma sodium during exercise in the heat. J Appl Physiol 1999 Jun; 86 (6): 1847–51

    PubMed  CAS  Google Scholar 

  67. Coyle EF. Fluid and fuel intake during exercise. J Sports Sci 2004 Jan; 22 (1): 39–55

    Article  PubMed  Google Scholar 

  68. Achten J, Jeukendrup AE. Effects of pre-exercise ingestion of carbohydrate on glycaemic and insulinaemic responses during subsequent exercise at differing intensities. Eur J Appl Physiol 2003 Jan; 88 (4-5): 466–71

    Article  PubMed  CAS  Google Scholar 

  69. Jentjens RL, Jeukendrup AE. Effects of pre-exercise ingestion of trehalose, galactose and glucose on subsequent metabolism and cycling performance. Eur J Appl Physiol 2003 Jan; 88 (4-5): 459–65

    Article  PubMed  CAS  Google Scholar 

  70. Jentjens RL, Cale C, Gutch C, et al. Effects of pre-exercise ingestion of differing amounts of carbohydrate on subsequent metabolism and cycling performance. Eur J Appl Physiol 2003 Jan; 88 (4-5): 444–52

    Article  PubMed  CAS  Google Scholar 

  71. Moseley L, Lancaster GI, Jeukendrup AE. Effects of timing of pre-exercise ingestion of carbohydrate on subsequent metabolism and cycling performance. Eur J Appl Physiol 2003 Jan; 88 (4-5): 453–8

    Article  PubMed  CAS  Google Scholar 

  72. Hargreaves M, Hawley JA, Jeukendrup A. Pre-exercise carbohydrate and fat ingestion: effects on metabolism and performance. J Sports Sci 2004 Jan; 22 (1): 31–8

    Article  PubMed  Google Scholar 

  73. Noakes TD, Rehrer NJ, Maughan RJ. The importance of volume in regulating gastric emptying. Med Sci Sports Exerc 1991 Mar; 23 (3): 307–13

    PubMed  CAS  Google Scholar 

  74. Goulet E, Gauthier P, Labrecque S, et al. Glycerol hyperhydration, endurance performance, and cardiovascular and thermoregulatory responses: a case study of a highly trained triathlete. J Exerc Physiol 2002 May; 5 (2): 19–28

    Google Scholar 

  75. Sommer S, Nau R, Wieland E, et al. Pharmacokinetics of glycerol administered orally in healthy volunteers. Arzneimittelforschung 1993 Jul; 43 (7): 744–7

    PubMed  CAS  Google Scholar 

  76. Shirreffs SM, Armstrong LE, Cheuvront SN. Fluid and electrolyte needs for preparation and recovery from training and competition. J Sports Sci 2004 Jan; 22 (1): 57–63

    Article  PubMed  Google Scholar 

  77. Massicotte D, Scotto A, Peronnet F, et al. Metabolic fate of a large amount of 13C-glycerol ingested during prolonged exercise. Eur J Appl Physiol 2006; 96: 322–9

    Article  PubMed  CAS  Google Scholar 

  78. Burelle Y, Massicotte D, Lussier M, et al. Oxidation of [(13)C]glycerol ingested along with glucose during prolonged exercise. J Appl Physiol 2001 May; 90 (5): 1685–90

    PubMed  CAS  Google Scholar 

  79. Noakes TD. Drinking guidelines for exercise: what evidence is there that athletes should drink “as much as tolerable”, “to replace the weight lost during exercise” or “ad libitum”? J Sports Sci 2007 May; 25 (7): 781–96

    Article  PubMed  CAS  Google Scholar 

  80. Shirreffs SM, Taylor AJ, Leiper JB, et al. Post-exercise rehydration in man: effects of volume consumed and drink sodium content. Med Sci Sports Exerc 1996 Oct; 28 (10): 1260–71

    Article  PubMed  CAS  Google Scholar 

  81. Arnall DA, Goforth Jr HW. Failure to reduce body water loss in cold-water immersion by glycerol ingestion. Undersea Hyperb Med 1993 Dec; 20 (4): 309–20

    PubMed  CAS  Google Scholar 

  82. O’Brien C, Freund BJ, Young AJ, et al. Glycerol hyperhydration: physiological responses during cold-air exposure. J Appl Physiol 2005 Aug; 99 (2): 515–21

    Article  PubMed  Google Scholar 

  83. Frank MS, Nahata MC, Hilty MD. Glycerol: a review of its pharmacology, pharmacokinetics, adverse reactions, and clinical use. Pharmacotherapy 1981 Sep-Oct; 1 (2): 147–60

    PubMed  CAS  Google Scholar 

  84. McCurdy DK, Schneider B, Scheie HG. Oral glycerol: the mechanism of intraocular hypotension. Am J Ophthalmol 1966 May; 61 (5): 1244–9

    PubMed  CAS  Google Scholar 

  85. Tourtellotte WW, Reinglass JL, Newkirk TA. Cerebral dehydration action of glycerol: I, historical aspects with emphasis on the toxicity and intravenous administration. Clin Pharmacol Ther 1972 Mar-Apr; 13 (2): 159–71

    PubMed  CAS  Google Scholar 

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Acknowledgements

The authors would like to acknowledge the University of Queensland Graduate School Research Travel Grant for financial assistance. The authors have no conflicts of interest that are directly relevant to the content of this review.

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van Rosendal, S.P., Osborne, M.A., Fassett, R.G. et al. Guidelines for Glycerol Use in Hyperhydration and Rehydration Associated with Exercise. Sports Med 40, 113–139 (2010). https://doi.org/10.2165/11530760-000000000-00000

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