001 AEROBIC EXERCISE TRAINING REDUCES PRE-BETA1 HDL GENERATION AND ENHANCES THE HDL₃ ANTIOXIDANT ROLE IN TYPE 2 DIABETES MELLITUS SUBJECTS

I. C. D. Ribeiro¹, R. T. Iborra¹, M. Q. T. S. Neves¹, S. A. Lottenberg¹, A. M. Charf¹, V. S. Nunes¹, C. E. Negrão², E. C. R. Quintão¹, E. R. Nakandakare¹, M. Passarelli¹.¹Lipids Laboratory (LIM-10), University of São Paulo Medical School, São Paulo, Brazil; ²Heart Institute, University of São Paulo Medical School, São Paulo, Brazil

Objectives: We analysed the influence of aerobic exercise training (AET) on plasma lipids, generation of pre-beta1 HDL, and HDL₃ ability to remove cell cholesterol (chol) and inhibit LDL oxidation.

Methods: Healthy controls (C, n = 11) and type 2 diabetes mellitus patients (DMT, n = 11) were engaged in AET for 4 months, and compared to sedentary DM (DMS, n = 10). All subjects were submitted at admission and after 4 months to an oral fat load test with blood drawn at fasting (F) and 2, 4, 6 (PP), and 8 h after a fat-rich meal. HDL₃ was isolated from F and PP plasmas and incubated with chol loaded cells to investigate chol efflux or with a healthy donor’s plasma LDL pool for measuring the lag time for LDL oxidation (LAG) on CuSO₄ at 37°C (234 nm). Pre-beta1 HDL was measured by ELISA.

Results: V̇O₂ maximal consumption was increased (10–20%) only in C and DMT. After AET, waist circumference was reduced, but there were no differences among the three groups regarding body weight, BMI, plasma total chol (TC), apoB, LDL-C, HDL-C, triglycerides (TG), glucose, insulin, HOMA-IR index, and areas under the curve of plasma TC, TG, and glucose. AET did not modify the F and PP composition of HDL₃ or its ability to remove cell chol. In DMT alone, pre-beta1 HDL was higher …