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THE EFFECT OF RELAXIN TREATMENT ON SKELETAL MUSCLE INJURIES
Professor Martin P Schwellnus, University of Cape Town, South Africa
Background: In the repair of injured skeletal muscle neutralisation of the effect of transforming growth factor-b1 (a key fibrotic cytokine) by the hormone relaxin may prevent fibrosis, increase muscle regeneration, and thereby improve the recovery following muscle injury.
Research question/s: Does the administration of relaxin, a member of the family of insulin-like growth factors, reduce fibrosis and improve the healing of injured muscle?
Methodology:Materials:Two studies: study 1 (in vitro), myoblasts (C2C12 cells) and myofibroblasts (transforming growth factor-β1-transfected myoblasts); study 2 (in vivo), 24 female mice.
Experimental procedure: In vitro study: myoblasts and myofibroblasts were incubated with relaxin and cell growth and differentiation were determined (myogenic and fibrotic protein expression).
In vivo study: relaxin was injected intramuscularly at different time points (none = control, days 3, 7 and 14) after the induction of a laceration injury in the skeletal muscle of the mice.
Measures of outcome: Skeletal muscle healing (histological, immunohistochemical, and physiologic parameters).
In vivo study: relaxin administrtion decreased myofibroblast proliferation, down-regulated expression of the fibrotic protein α-smooth muscle actin in a dose-dependent manner.
In vitro study: relaxin administration enhanced muscle regeneration, reduced fibrosis, and improved injured muscle strength.
Conclusion/s: In an …
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