Aim To quantify the year-to-year variability of altitude-induced changes in haemoglobin mass (Hb_mass) in elite team-sport athletes.

Methods 12 Australian-Footballers completed a 19-day (ALT1) and 18-day (ALT2) moderate altitude (∼2100 m), training camp separated by 12 months. An additional 20 participants completed only one of the two training camps (ALT1 additional n=9, ALT2 additional n=11). Total Hb_mass was assessed using carbon monoxide rebreathing before (PRE), after (POST₁) and 4 weeks after each camp. The typical error of Hb_mass for the pooled data of all 32 participants was 2.6%. A contemporary statistics analysis was used with the smallest worthwhile change set to 2% for Hb_mass.

Results POST₁ Hb_mass was very likely increased in ALT1 (3.6±1.6%, n=19; mean±∼90 CL) as well as ALT2 (4.4±1.3%, n=23) with an individual responsiveness of 1.3% and 2.2%, respectively. There was a small correlation between ALT1 and ALT2 (R=0.21, p=0.59) for a change in Hb_mass, but a moderately inverse relationship between the change in Hb_mass and initial relative Hb_mass (g/kg (R=−0.51, p=0.04)).

Conclusions Two preseason moderate altitude camps 1 year apart yielded a similar (4%) mean increase in Hb_mass of elite footballers, with an individual responsiveness of approximately half the group mean effect, indicating that most players gained benefit. Nevertheless, the same individuals generally did not change their Hb_mass consistently from year to year. Thus, a ‘responder’ or ‘non-responder’ to altitude for Hb_mass does not appear to be a fixed trait.