Background Football is the most popular sport in the world, including in Iran. Muscular injuries has 31 percent of all injuries and due to this injury, 27 percent of athletes couldn’t contribute at sport sessions.

Objective To predict selected performance and skill test batteries of severity of injury in Iran premier league football players.

Design Prospective cohort study, purposeful sampling method for selecting football players.

Setting Youth male football players of Iran premier league.

Patients (or Participants) A total of 79 premier league football players aged 16–21 years old who were member of two professional football clubs were selected for the study.

Interventions (or Assessment of Risk Factors) At the pre-season time of league, five performance tests including Standing Long Jump Test, Change of Direction and Acceleration Test(CODAT), Illinois Agility Test, Yoyo Intermittent Recovery Test and Y-Balance Test (YBT), and one skill test including Loughborough Soccer Shooting Test were performed. The pre-season medical assessment forms including the number of players injuries were collected by the club medical staff. This information were gathered weekly and were sent to the researcher.

Results Logistic regression analysis results showed that, of performance and skill tests, just Yoyo Intermittent Recovery Test could predicted the severity of injury (P<0.05). Investigation of predictive variable index was showed that the Wald test of performance and skill test was significant just for Yoyo Intermittent Recovery Test and was able to predict injury severity.

Conclusions None of the performance and skill tests could predict the severity of injury, except the Yoyo intermittent recovery test. This test were designed for measurement of aerobic capacity of athletes. We suggest that coaches use this test to identify at-risk athletes.