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Chronic dynamic exercise increases Apolipoprotein A-I expression in rabbit renal cortex as determined by Proteomic technology
  1. Roger de Moraes (etibi{at}
  1. Oswaldo Cruz Institute, Brazil
    1. Richard Hemmi Valente (rhv4u{at}
    1. Oswaldo Cruz Institute, Brazil
      1. Ileana Rodrigues Leon (ileana{at}
      1. Oswaldo Cruz Institute, Brazil
        1. Monique Trugilho (monique{at}
        1. Oswaldo Cruz Institute, Brazil
          1. Jonas Perales (jperales{at}
          1. Oswaldo Cruz Institute, Brazil
            1. Antônio Cláudio Lucas Nobrega (anobrega{at}
            1. Fluminense Federal University, Brazil
              1. Eduardo Tibirica (etibi{at}
              1. Oswaldo Cruz Institute, Brazil


                Objective: We demonstrated previously that exercise training enhances endothelium-dependent and -independent rabbit kidney vascular relaxation. The present study investigated protein expression changes in the rabbit renal cortex induced by chronic dynamic exercise.

                Design: Kidneys were obtained from New Zealand rabbits either pen confined (n=8) or treadmill trained (0% grade) for 5 days/wk at a speed of 18 m/min during 60 min over a 12-week period (n=8). Expression of proteins in the renal cortex was determined by colloidal Coomassie blue staining after two-dimensional polyacrylamide gel electrophoresis. Differencial protein spots were excised, trypsin digested and peptides sequenced by electrospray ionization-ion trap mass spectrometry.

                Results: Two pairs of matching differentially stained spots displayed an approximate 3-fold increase in trained animals when compared to sedentary ones. These four spots presented a molecular mass of 23 kDa but different pI values. Mass spectrometric analyses revealed the pairs of matching spots as being rabbit apolipoprotein A-I.

                Conclusion: Chronic dynamic exercise increases apolipoprotein A-I expression in the rabbit renal cortex. This fact could be involved in the alterations observed in the renal circulation after exercise training.

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