Article Text

How does hormone transition in transgender women change body composition, muscle strength and haemoglobin? Systematic review with a focus on the implications for sport participation
  1. Joanna Harper1,
  2. Emma O'Donnell1,
  3. Behzad Sorouri Khorashad2,
  4. Hilary McDermott1,
  5. Gemma L Witcomb1
  1. 1School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK
  2. 2Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden
  1. Correspondence to Dr Gemma L Witcomb, School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK; G.L.Witcomb{at}


Objectives We systemically reviewed the literature to assess how long-term testosterone suppressing gender-affirming hormone therapy influenced lean body mass (LBM), muscular area, muscular strength and haemoglobin (Hgb)/haematocrit (HCT).

Design Systematic review.

Data sources Four databases (BioMed Central, PubMed, Scopus and Web of Science) were searched in April 2020 for papers from 1999 to 2020.

Eligibility criteria for selecting studies Eligible studies were those that measured at least one of the variables of interest, included transwomen and were written in English.

Results Twenty-four studies were identified and reviewed. Transwomen experienced significant decreases in all parameters measured, with different time courses noted. After 4 months of hormone therapy, transwomen have Hgb/HCT levels equivalent to those of cisgender women. After 12 months of hormone therapy, significant decreases in measures of strength, LBM and muscle area are observed. The effects of longer duration therapy (36 months) in eliciting further decrements in these measures are unclear due to paucity of data. Notwithstanding, values for strength, LBM and muscle area in transwomen remain above those of cisgender women, even after 36 months of hormone therapy.

Conclusion In transwomen, hormone therapy rapidly reduces Hgb to levels seen in cisgender women. In contrast, hormone therapy decreases strength, LBM and muscle area, yet values remain above that observed in cisgender women, even after 36 months. These findings suggest that strength may be well preserved in transwomen during the first 3 years of hormone therapy.

  • body composition
  • gender issues
  • physiology
  • psychology
  • strength

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Currently the world of sport, from grassroots level to elite, is facing the challenge of how to include transgender people in sporting competitions. Regulations governing the participation of athletes from outside the sex/gender binary have existed since the 1940s.1–4 Presently, World Athletics requires that transgender athletes5 and athletes with differences of sexual development6 have testosterone levels ≤5 nmol/L in order to be eligible for the female category. There has been heavy criticism of this, and previous, testosterone-based regulations.7–9 Although no openly transgender athlete has competed in the Olympics to date, the increasing visibility of gender-diverse people in society10 means that the sports administrators and legislators must create rules to accommodate athletes from outside the sex/gender binary.11

There are many quantifiable performance-related differences between male and female athletes. In contrast, the performance-related differences between transwomen who have received gender affirming hormone treatment (GAHT) and cisgender women are less clear. GAHT for transwomen consists of an antiandrogen agent plus the introduction of exogenous oestrogen,12 with the goal of altering the hormonal milieu and, as a result, feminisation of the body.13 To date, there have been no prospective studies investigating the changes in athletic performance in transgender athletes after hormonal transition. In non-athletic transgender populations, studies are commonly focused on clinical outcomes, such as bone health.14 However, studies in non-athletic transwomen undergoing GAHT also report changes in lean body mass (LBM),15 muscle cross-sectional area (CSA),16 muscular strength17 and haemoglobin (Hgb)18 and/or haematocrit (HCT).19 These parameters are of relevance to athletic performance.

In endurance sports, Hgb is of importance. Hgb is a protein carried by the red blood cells that is responsible for transporting oxygen from the lungs to peripheral tissues.20 Low Hgb, or low HCT, the volume of red blood cells compared with total blood volume, can lead to a diminished supply of oxygen to the tissues, and therefore have a direct effect on endurance performance. Typical values for Hgb differ between males and females, with ‘normal’ values ranging between 131–179 g/L for men and 117–155 g/L for women.21 HCT values are also higher in males (42%–52%) than females (37%–47%).22 Testosterone exerts erythrogenic effects that results in increases in both HCT and Hgb.23 Since GAHT significantly lowers testosterone levels in transgender women,24 it is possible that they may experience reductions in HCT and Hgb, which would be anticipated to negatively affect endurance performance.

In sports demanding speed and power, muscular strength and the ability to generate high rates of force are recognised as key determinant in athletic success.25 In cisgender males, increases in testosterone due to puberty promote muscular strength in association with increased muscle CSA, and increased lean muscle mass.26 It has been hypothesised that muscle retains a long-term memory allowing it to perform tasks that it has undertaken many times previously and myonuclei retention is thought to play an important role in such muscle memory.27 Myonuclei number is increased with training and with use of anabolic steroids.28 However, detraining does not diminish the myonuclei number,27 and it has been hypothesised that cessation of steroids may also not lead to reductions in myonuclei number.28 Hence, it is possible that strength advantages gained when training in a high-testosterone environment may not be fully reversed by testosterone suppression.

Understanding both the physiological effects of GAHT on athletic performance, and the time course of these effects, is of importance to decision-makers and those undertaking policy reviews. While it is known that testosterone levels are markedly reduced in transgender women taking testosterone suppressing GAHT,29 the effects of this hormonal change on physiology, and the time course in which these changes occur, are less clear. Individual studies provide crucial, primary research on the topic, but a systematic review is warranted to provide a robust summary of the available evidence. Because bone mineral density studies have already been subject to systematic review,30 31 this review focuses on physiological changes induced by GAHT in transwomen that affect athletic performance; specifically, LBM, CSA, strength and Hgb/HCT.


The aim of this systematic review was to: (1) summarise the current state of knowledge as it relates to the changes, and the time course of these changes, in physiological parameters associated with athletic performance in non-athletic transwomen resulting from GAHT (suppression of testosterone and supplementation with oestrogen), and (2) consider the potential implications for the participation of transwomen in elite sport.

Materials and methods

Search strategy and selection criteria

This systematic review was conducted in line with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.32 Two electronic searches of four online databases (BioMed Central, PubMed, Scopus and Web of Science) were completed 15 months apart. The first was performed by BSK in January 2019 and the second by JH in April 2020. The two sets of search results were compared by GLW. The second search identified novel data from three additional studies using the same cohorts as three studies identified in the first search. The more recent search also identified three additional recent papers. Reference lists were also searched for additional citations pertinent to the review. The searches combined terms related to transwomen, GAHT, muscle and blood parameters (online supplemental table 1).

Study selection, quality assessment, and data extraction

Each study was initially categorised based on its design (eg, cohort, case–control) and examined for quality in line with the Effective Public Health Practise Project (EPHPP) tool.33 This is a generic tool used to evaluate a variety of intervention study designs and is suitable for use in systematic reviews,34 having content and construct validity.35 Based on the EPHPP, six domains are evaluated: (1) selection bias; (2) study design; (3) confounders; (4) blinding; (5) data collection method; and (6) withdrawals/dropouts. Each domain is rated as strong (3 points), moderate (2 points) or weak (1 point), and domain scores are averaged to provide the overall mean rating. Based on the overall mean rating, studies are rated as weak (1.00–1.50), moderate (1.51–2.50) or strong (2.51–3.00).

For longitudinal studies, data were extracted to examine changes in LBM, CSA, strength and Hgb/HCT in transwomen taking GAHT. In cross-sectional studies, data in transwomen were compared with data from both cisgender men and cisgender women. The study authors were contacted if there were any questions regarding the presented data. In this regard, authors of the nine studies carried out by the European Network for the Investigation of Gender Incongruence (ENIGI) were contacted regarding potential overlapping participants15 17 19 36–41 and another author was contacted to clarify graphical data content.16


Search results

Figure 1 shows the search strategy following PRISMA guidelines. From an initial yield of 795 articles, 24 studies15–19 36–54 were included in this review. The following information was extracted from each study: name of the first author, country, year of publication, number of transfemale participants, number of cisgender male and female participants (where applicable), duration of any follow-up, type of medical treatment, method of measurement, evaluation time, and results.

Figure 1

PRISMA flow chart illustrating search strategy. PRISMA, Preferred Reporting Items for Systematic Reviews and Meta-Analyses.

Quality assessment

Based on the mean EPHPP scores, all studies were categorised as moderate in quality. The individual scores are listed in the online supplemental table 2.

Study characteristics

A summary of the study characteristics is reported in table 1. The sample sizes of the studies varied from 12 to 249. Three large studies from the ENIGI group published in 2018 and 201915 17 19 contained much novel data, but also included many participants from previous studies making it impossible to accurately state the number of unique participants.

Table 1

Characteristics of reviewed studies

Study designs

Thirteen studies15 17 19 36–40 42 43 46–48 utilised a follow-up study design comparing participants’ measurements before initiating hormone transition (baseline) to several months after hormone transition. Two studies41 51 used both follow-up and cross-sectional designs with cisgender controls. Six studies18 45 50 52–54 used an exclusively cross-sectional design; three comparing transwomen on GAHT with cisgender controls18 53 54 and three comparing transwomen on GAHT with hormone-naive transwomen.45 50 52 Three studies16 44 49 used a prospective method gathering data over 12–24 months. Aside from these three studies, data were extracted from medical charts (nine of which were from the same research group,15 17 19 36–41) posing a risk of selective data reporting and publication bias.

Medical treatments

Medical treatments for endocrine transition were varied, in line with the individualised approach advised by the WPATH Standards of Care.55 Fourteen studies15 17 19 36–43 46 48 54 used cyproterone acetate (50–100 mg daily) as an antiandrogen. In six studies16 38 40 44 46 49 a form of gonadotropin-releasing hormone agonist was administered either to supress puberty or androgens. In four studies18 49 50 52 spironolactone was used as an antiandrogen. Seventeen studies15 17–19 36–39 41 44 45 47–50 52 53 used 2–4 mg/day of oral oestradiol valerate. Eleven studies15–17 19 39 42 43 45 46 48 49 used transdermal 17-beta-oestradiol releasing 100 mcg/day. Four studies16 18 47 49 used an injection of oestradiol valerate (10 mg/ampoule, every 1–4 months). Two studies45 54 used 0.625–2.5 mg/day of conjugated equine oestrogen. Four studies,42 43 51 54 all undertaken prior to 2010, used 25–50 mcg/day of ethinyl oestradiol. Ethinyl oestradiol was not used in any study after 2010, primarily due to increased risk of thrombogenesis.56

Based on the variability in drug regimens used, there is substantial heterogeneity in the hormone levels achieved. Although the transwomen in most of the studies achieved testosterone levels within the reference range for cisgender women, there were five studies38 40 47 49 51 in which the transfemales had post-GAHT testosterone values greater than 5 nmol/L. Four of the five studies38 40 47 49 were carried out on adolescent transfemales; two of the five studies38 51 did not involve the use of an antiandrogen agent; one study40 did not involve the use of any form of oestrogen. The high post-GAHT testosterone is a possible confounder, and potential physiological differences between adolescent and adult participants may also confound results.

Muscle mass and body fat changes

Table 2 summarises the studies reporting muscle mass and body fat. Eight studies15 36 39–41 44 46 51 used a follow-up design to assess changes in LBM; seven studies assessed after 12 months,15 36 39 41 44 46 51 and one40 study reviewed patients who had been under treatment for 5–31 months. Seven of these studies,15 36 39–41 44 51 including the large (n=179) ENIGI study,15 and two studies40 51 with high post-GAHT testosterone (~8 nmol/L), showed that total LBM was decreased by 3.0%–5.4% following hormone transition (p<0.05). The one study that failed to demonstrate significant changes in LBM46 was not an outlier in any obvious way. The large ENIGI study15 was the only study in which the limits of agreement would indicate a change in LBM at the 95% CI. All studies reported an increase in total body fat mass in transwomen after hormone transition. Three cross-sectional studies41 51 54 compared transwomen with cisgender men. Two studies included hormone-naive transwomen.41 51 These studies reported 6.4% and 8.0% lower LBM than in cisgender men and reductions of 4% in LBM in the transwomen with 12 months of GAHT. The third cross-sectional study compared transwomen who had undergone at least 48 months of GAHT with cisgender men54 and reported 17% lower LBM in transwomen than in cisgender men.

Table 2

Changes in total LBM in kilograms

CSA changes

Four follow-up studies16 40–42 investigated the CSA either in the quadriceps, forearm or calf regions using MRI16 42 or peripheral quantitative computed tomography (pQCT).40 41 Of note, two of the studies measured the total CSA of the individual MRI42 or pQCT41 image while two studies measured the isolated muscle.16 40 A decrease in CSA of 1.5%–11.7% was reported over periods ranging from 12 to 36 months. One of these studies40 examined adolescent participants who only reached a final testosterone level of 8.8 nmol/L and exhibited forearm and calf CSA decreases of 4.1% and 8.9%, respectively. There were two studies41 42 that assessed muscle CSA at both 12 months and at either 24 or 36 months. The first study42 reported a 9.5% decrease in quadriceps CSA compared with baseline after 12 months and an 11.7% decrease in quadriceps CSA compared with baseline after 36 months. The second study41 reported a 1.5% decrease in tibia CSA compared with baseline after 12 months and a 3.8% decrease compared with baseline after 24 months. The same study reported that compared with baseline, forearm CSA was decreased by 8.6% after 12 months, yet at 24 months was 4.4% lower than baseline, indicating that forearm CSA was 4.2% larger at 24 months than at 12 months. There was only one study42 in which the limits of agreement indicated a change at the 95% CI. Two cross-sectional studies41 54 compared transwomen with cisgender men. One study reported 9% smaller CSA in hormone-naive transwomen41 than in cisgender men, with the transwomen undergoing a further 4% decrease in CSA with 24 months of GAHT. The transwomen in the second study had all undergone at least 48 months of GAHT54 and had 24% smaller CSA than cisgender men. See table 3.

Table 3

Changes in muscle CSA

Muscular strength changes

Table 4 summarises the studies reporting muscular strength. Five longitudinal studies16 17 37 40 41 investigated the muscular strength of transwomen. Four of the studies17 37 40 41 measured hand grip strength in participants on the ENIGI study. The largest of the three (n=249) ENIGI studies17 and one other study41 found significant (p<0.001) reductions (4.3% and 7.1%, respectively) after 12 months on GAHT. Two ENIGI studies37 40 found no significant strength differences, although one of these studies40 was carried out on adolescents who failed to reach typical female testosterone levels (8.8 nmol/L after GAHT). The large ENIGI study17 was the only study in which the limits of agreement would indicate a change in strength at the 95% CI. The fifth longitudinal study to assess strength measured upper leg strength using knee flexion and extension and found no significant difference after 12 months.16 Two studies41 54 used a cross-sectional design to compare the strength of transwomen to cisgender men. One study found 14% lower hand grip strength in hormone-naive transwomen than in cisgender men (p<0.001)41 and a further 7% reduction in hand grip strength of the transwomen after 12 months of GAHT. The other study54 found 24% lower hand grip and quadriceps strength in transwomen than in cisgender men after 48 months or more on GAHT (p<0.001).

Table 4

Changes in strength measures

Hgb and HCT changes

Nine studies16 19 36–38 43 47–49 reported the levels of Hgb or HCT in transwomen before and after GAHT, from a minimum of three to a maximum of 36 months post hormone therapy. Eight of these studies,16 19 36–38 43 48 49 including the large (n=239) ENIGI study,19 found that hormone therapy led to a significant (4.6%–14.0%) decrease in Hgb/HCT (p<0.01), while one study found no significant difference after 6 months.47 The mean age of participants in the latter study was 18 years and the range was 14–25 years. The participants also failed to reach typical female testosterone levels (after 6 months mean testosterone=6.9 nmol/L), while in six16 19 36 37 43 48 of the eight other studies mean testosterone after GAHT was less than 2.0 nmol/L. The large ENIGI study19 was the only study in which the limits of agreement would indicate a change in Hgb/HCT at the 95% CI. Three cross-sectional studies18 53 54 compared HCT in transwomen post GAHT with cisgender controls (table 5). Two studies found that transwomen on GAHT for 6 or 48 months had lower (10%) HCT than cisgender men53 54 (p<0.005), while two studies found no difference between transwomen after 6 and 12 months of GAHT and cisgender women.18 53 Three cross-sectional studies45 50 52 found significant differences45 50 (p<0.05) or large effect sizes52 (Cohen’s d=1.0) in HCT between transwomen after 6 months of GAHT and hormone-naive transwomen, and HCT decreases of 7.4%–10.9%. See table 5.

Table 5

Changes in HCT and Hgb levels


We summarise changes induced by GAHT in non-athletic transwomen in four characteristics strongly associated with athletic performance: LBM, muscle CSA, muscular strength, and Hgb/ HCT levels. Overall, the findings demonstrate a reduction in these parameters over time. However, the time course of these reductions was not consistent across the parameters assessed.

In keeping with the muscular anabolic effects of testosterone57 and the mixed effects of oestrogens,58 studies using dual energy X-ray absorptiometry report decreased LBM (0.8%–5.4%) in association with GAHT. Twelve months of GAHT also decreased muscle CSA (1.5%–9.7%). However, a further 12 or 24 months of GAHT did not always elicit further decreases in muscle CSA. Strength loss with 12 months of GAHT also ranged from non-significant to 7%. Taking these strength parameter data collectively, and in consideration of cisgender women demonstrating 31% lower LBM,59 36%60 lower hand-grip strength and 35%61 lower knee extension strength than cisgender men, the small decrease in strength in transwomen after 12–36 months of GAHT suggests that transwomen likely retain a strength advantage over cisgender women. Whether longer duration of GAHT would yield further decrements in strength in transgender women is unknown.

In contrast to strength-related data, blood cell findings revealed a different time course of change. After 3–4 months on GAHT, the HCT19 or Hgb16 levels of transwomen matched those of cisgender women, with levels remaining stable within the ‘normal’ female range for studies lasting up to 36 months. Given the rapid fall in Hgb/HCT to ‘normal’ female levels with GAHT, it is possible that transfemale athletes experience impaired endurance performance in part due to reduced oxygen transport from the lungs to the working muscles.62 This postulate is consistent with findings reported in one of the few studies conducted in athletic transwomen.63 In this study, the race times of eight transfemale distance runners were compared at baseline and after one or more years of GAHT. After adjusting performance for age, the eight runners were not more competitive in the female category (after GAHT) than they had been in the male category (before GAHT). Given this, and that the changes in Hgb/HCT follow a different time course than strength changes, sport-specific regulations for transwomen in endurance ver strength sports may be needed.

Of interest, compared with cisgender men, hormone-naive transwomen demonstrate 6.4%–8.0% lower LBM,41 51 6.0%–11.4% lower muscle CSA and ~10%–14% lower handgrip strength.17 41 60 This disparity is noteworthy given that hormone-naive transwomen and cisgender men have similar testosterone levels.16 17 19 42 Explanations for this strength difference are unclear but may include transwomen actively refraining from building muscle and/or engaging in disordered eating64 or simply not being athletically inclined, perhaps influenced by feelings of an unwelcome presence in sporting arenas.65 Taken together, hormone-naive transwomen may not, on average, have the same athletic attributes as cisgender men. The need to move beyond simple comparisons of cisgender men and women to assess the sporting capabilities of transwomen is imperative.

This systematic review identified studies that assessed the changes in LBM, CSA, muscular strength and Hgb/HCT in non-athletic transgender women following GAHT. However, several limitations are noted. Although the data we present are meaningful, the effects of GAHT on these parameters, or indeed athletic performance in transgender people who engage in training and competition, remain unknown. The levels of physical activity of the transwomen compared with cisgender women in the studies were not reported. Other limitations include the studies being written in English only, and the research being conducted in Western countries, contributing to geographical bias. Furthermore, as with much research with transgender individuals, there is a sparse data risk66 because of small sample sizes and short study durations, indicative of the relatively small population, difficulties with recruitment and high drop-out rates over time. Indeed, the overlap of participants in the ENIGI studies and the heterogenous methodology in the other studies precluded the possibility of meaningful meta-analysis. However, overall, the results across different study groups and methods (ie, longitudinal vs follow-up studies) are largely consistent, suggesting that the risk of selective reporting and publication bias are low and the data in the reviewed studies are reliable. This review only focused on binary transgender individuals; those who medically transition from their birth assigned gender to the opposite gender and did not consider non-binary individuals. Not only are there even more limited data on non-binary individuals, but also, for many, their affirmed gender expression does not require GAHT, thus there are no hormone-induced changes to observe which would be relevant to this review. That is not to say that non-binary inclusivity in sport is not an important issue, only that the central tenets are not focused on physiology.

As previously stated, a major limitation in this area of research is the absence of studies in transgender athletes. However, a very recent study reported changes in fitness levels of 29 transmen and 46 transwomen in the United States Air Force, from before and after 30 months of GAHT.67 Enlisted Air Force members are required to engage in regular physical activity and to complete annual assessments of number of sit-ups and push-ups in 1 min, and 1.5 mile race time. Although not athletes per se, enlisted members could at least be considered exercise trained. The study reported that after 2 years on GAHT there were no significant differences between ciswomen and transwomen in the number of push-ups or sit-ups performed in 1 min. However, transwomen ran significantly faster during the 1.5 mile fitness test than ciswomen. These observations in trained transgender individuals are consistent with the findings of the current review in untrained transgender individuals, whereby 30 months of GAHT may be sufficient to attenuate some, but not all, influencing factors associated with muscular endurance and performance.

Overall, this review reports decreases in muscle strength, LBM and muscle CSA in response to 12–36 months, and decreases in Hbg after 3–4 months, of GAHT in transwomen. These findings may help to shape future studies with transgender athletes and provide data for valuable and rigorous research going forward. Sporting bodies wish to be inclusive to all athletes, and there is a critical desire and need for more research to be able to develop evidence-based policies around this topic. Given that the range of physical parameters important for success varies considerably between sports, and that the physiological effects of GAHT vary in their time course (eg, muscle vs blood), future research should be sport specific as well as athlete centric. Although a level playing field in sport is illusory, it is important that opportunities for women to engage in meaningful competition within the female category exist.68 Whether transgender and cisgender women can engage in meaningful sport, even after GAHT, is a highly debated question. However, before this question can be answered with any certainty, the intricacies and complexity of factors that feed into the development of high-performance athletes warrant further investigation of attributes beyond those assessed herein.

What is already known

  • There is much debate on whether (and when) transwomen should be permitted to compete in the female category in sport.

What are the new findings

  • Longitudinal and cross-sectional studies identify that hormone therapy in transwomen decreases muscle cross-sectional area, lean body mass, strength and haemogloblin levels, with noted differences in the time course of change.

  • Haemoglobin levels decrease to those seen in cisgender women after 4 months of hormone therapy. In contrast, despite significant decreases in muscle cross-sectional area, lean body mass and strength after 12–36 months of hormone therapy, values remain higher than that in cisgender women.

  • It is possible that transwomen competing in sports may retain strength advantages over cisgender women, even after 3 years of hormone therapy.


Supplementary materials

  • Supplementary Data

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  • Contributors GLW devised the study. BSK completed an initial search in 2019 with GLW and HMD. JH completed a second search in 2020 with GLW and EOD. All authors contributed to the manuscript.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

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