@article {Kicman253, author = {A T Kicman and R V Brooks and S C Collyer and D A Cowan and M N Nanjee and G J Southan and M J Wheeler}, title = {Criteria to indicate testosterone administration.}, volume = {24}, number = {4}, pages = {253--264}, year = {1990}, doi = {10.1136/bjsm.24.4.253}, publisher = {British Association of Sport and Excercise Medicine}, abstract = {A detection method for testosterone administration was developed using radioimmunoassay to measure the urinary ratios of testosterone (T) to epitestosterone (E) and to luteinizing hormone (LH). A comparative study of the effect on these ratios of a single intramuscular injection of testosterone heptanoate followed by stimulation with human chorionic gonadotrophin (HCG) in three normal men was undertaken. To allow immediate investigation, a commercially supplied epitestosterone antiserum was used. This study showed that both T/E and T/LH ratios could be used to detect testosterone administration, the latter also being an indicator of HCG use due to cross-reactivity with the LH antiserum. Subsequently, an epitestosterone antiserum of superior specificity was raised and used in a study to demonstrate the insignificant effect of exercise on these ratios. Finally, an intramuscular injection of a combined preparation of testosterone/epitestosterone heptanoates resulted in raised ratios of T/LH but not of T/E. This demonstrated the importance of the T/LH ratio in circumstances where the T/E ratio can be easily circumvented.}, issn = {0306-3674}, URL = {https://bjsm.bmj.com/content/24/4/253}, eprint = {https://bjsm.bmj.com/content/24/4/253.full.pdf}, journal = {British Journal of Sports Medicine} }