PT  - JOURNAL ARTICLE
AU  - A Scott
AU  - Ø Lian
AU  - R Bahr
AU  - D A Hart
AU  - V Duronio
AU  - K M Khan
TI  - Increased mast cell numbers in human patellar tendinosis: correlation with symptom duration and vascular hyperplasia
AID  - 10.1136/bjsm.2007.040212
DP  - 2008 Sep 01
TA  - British Journal of Sports Medicine
PG  - 753--757
VI  - 42
IP  - 9
4099  - http://bjsm.bmj.com/content/42/9/753.short
4100  - http://bjsm.bmj.com/content/42/9/753.full
SO  - Br J Sports Med2008 Sep 01; 42
AB  - Objectives: The cellular basis of painful tendon overuse pathology (tendinosis) is poorly understood. It has been suggested that because of the close anatomical associations between mast cells and vessels in connective tissues, mast cells may mediate the development of tendon hypervascularity or oedema.Objectives: To examine the distribution of mast cells in men and women with patellar tendinopathy.Design: Case–control study.Methods: Tendinopathic tissue was collected at open debridement of the patellar tendon and a control tendon from patients undergoing intramedullary nailing of the tibia. The tendon was assessed immunohistochemically by evaluating the distribution of mast cells (AA1), and markers for T lymphocytes (CD3) and macrophages (CD68). The vessel-area fraction was quantified using computer-assisted digital image analysis.Results: The prevalence of mast cells per mm2 (mean 3.3 (SD 3.0)) was greater in tendinosis tissue than in controls (1.1 (1.5); p = 0.036). In patients with tendinosis, mast cell density was moderately correlated with the vessel-area fraction (r2 = 0.49) and with symptom duration (r2 = 0.52).Conclusion: Mast-cell prevalence in patellar tendinopathy was increased and was predominantly associated with vascular hyperplasia, particularly in patients with longstanding symptoms. Future research should investigate whether mast cells play direct or indirect modulatory roles in the development and progression of human tendinosis.