TY - JOUR T1 - 3 Intrasound Therapy Reverses The Effects Of Alcohol Consumption On The Healing Tendon And Augments Healing JF - British Journal of Sports Medicine JO - Br J Sports Med SP - A2 LP - A3 DO - 10.1136/bjsports-2014-094114.3 VL - 48 IS - Suppl 2 AU - Ayoola I Aiyegbusi AU - Olufunke O Dosumu AU - Titilola A Samuel AU - Charles C Anunobi AU - Francis I Duru AU - Adegoke Akinfeleye Y1 - 2014/09/01 UR - http://bjsm.bmj.com/content/48/Suppl_2/A2.abstract N2 - Functional abnormalities in wound structure has been identified as a potential complication if a patient consumes alcohol prior to injury and studies suggest that even a single incidence of acute ethanol exposure can perturb the tissue response to trauma significantly. Ethanol ingestion has been shown to result in delayed and abnormal tendon healing 3 weeks after injury. This study investigated the effects of low intensity intrasound therapy (LITR) given twice daily on the morphology and antioxidant parameters in the healing tendon following an acute injury in rats exposed to prior ethanol consumption. Fifteen male rats, randomised into three groups all underwent induced crush injury to the Achilles tendon. Groups 2 and 3 had prior administration of 30% ethanol for six days. The three groups were allocated to: serve as controls (Group 1), received no treatment (Group 2), LITR twice daily (Group 3). LITR was commenced immediately post-injury and was given twice daily over the first 6 days. The animals were sacrificed on day 20 post-injury and the tendons were excised, and processed for histology and anti-oxidant and MDA assay. The tendons in group 2 showed disordered and haphazard collagen formation with neutrophilic infiltrates and high tenoblast population at 20 days post-injury while the LITR treated tendon had dense, well-ordered, parallel collagen deposits with fewer tenoblasts. LITR also significantly improved the antioxidant parameters and lowered the MDA compared with the alcohol-exposed untreated tendon (p < 0.05). LIRT thus reverses the deleterious effect of ethanol on the healing tendon and resulted in near-normal morphology of the healing tendon 20 days post-injury. Abstract 3 Figure 1 L/S of tendon of rat. A: control, B: alcohol fed with no treatment and C: alcohol fed and treated with LITR. (H&E) X200. A shows moderately aligned collagen fibres (C), B shows disorganized collagen fibres (C) with neutrophilic infiltrates (N) while C shows orderly and parallel collagen deposits (C) and elongated tenocytes (T) View this table:Abstract 3 Table 1 Correlation of antioxidants with MDA View this table:Abstract 3 Table 2 Population of tendon cells References Aiyegbusi et al . Connective Tissue Research. 2012;53(6):478–484 Novasonic. www.novasonicwest.com/novasonic. 2007 Molina et al. Alcohol Clin Exp Res. 2002;26(1):120–8 Sommers MS. Crit Care Nurse. 1994;14:82–86, 88–93 ER -