Table 1 Primer pair sequences used to amplify the SNP within the 3′-UTR of the COL5A1 gene
Variant (SNP)Oligonucleotide (primers)Sequences (5′–3′)Amplicon sizes
rs10858286Primer 1AGCAGTCAGCAGCGTGGGTCTGGTTATCTControl 291 bp
Primer 1BTTTGGGGTGGCACTTGCAGCACTGGTCGT allele 207 bp
Primer 1CAAGCTGGCACTTCTGGGGTGAGATCAGGC allele 139 bp
Primer 1DGGCTGCTTCTCCTCTCCCTGCCCAGAGT
rs13946Primer 2AGTGAGTGTCCCGTGCACGTCCTTATControl 363 bp
Primer 2BGGGAGGGAGAAGCCTTCACTGTCCACGGC allele 248 bp
Primer 2CGGCTACCAGAAGACGGTTCTGGAGATCGT allele 167 bp
Primer 2DGCAGCTAGGTCATGTGATTCAGCTCCAG
rs4504708Primer 5AATACAATGTCAAATGACACATTGTATGGControl 370 bp
Primer 5BATGACATGTCTAGCGGATTTTTTGATAAT allele 234 bp
Primer 5CCCAGACCACTCTAGCCACAGTATATTGCG allele 191 bp
Primer 5DGAGAGTCACTCACATCTGTTTACGGTGG
rs3128575Primer 4AACACCCAACTCGATTTATTGAAAAAATGControl 394 bp
Primer 4BGTGTAACAAAGGAATAATTAACTGTCATT allele 252 bp
Primer 4CAGCTGTCCATGCCAGCCTTGGCCACTCCC allele 197 bp
Primer 4DCCAAGATAAAGTTTCGAGGACATCAGGC

  • SNP, single nucleotide polymorphism; UTR, untranslated region.