Data representing the basic histological characteristics and quantified immunohistochemistry of tissue viability in format p50 (p25–p75)
| RCR 0 weeks | RCR 7 weeks | GCI 0 weeks | GCI 7 weeks | |
|---|---|---|---|---|
| Median vessel count | 0.05 (0–0.35)**† | 1.04 (0.78–1.23)** | 0.36 (0.24–0.38)† | 0.42 (0.34–0.60) |
| Median nuclei count | 8.5 (2.7–16.6)** | 26.7 (21.5–45.2)** | 13.8 (10.9–16.9) | 16.5 (12.1–26.1) |
| Median SD of nuclei count | 6.7 (2.6–12.9)** | 20.4 (16.1–26.0)** | 11.1 (7.5–15.4) | 12.2 (9.1–16.1) |
| Median cell nuclear area (%) | 0.12 (0.09–0.14) | 0.12 (0.09–0.14) | 0.11 (0.07–0.13) | 0.11 (0.09–0.13) |
| Proliferation (% PCNA staining per cell) | 0.07 (0.01–0.21)* | 0.42 (0.23–0.58)* | 0.05 (0.02–0.12) | 0.06 (0.03–0.08) |
| p53 (% staining per cell) | 0.01 (0.001–0.02)* | 0.22 (0.09–0.22)* | 0.03 (0.01–0.1)* | 0.08 (0.02–0.23)* |
| HIF-1α (% staining per cell) | 0.03 (0.01–0.09)* | 0.11 (0.02–0.40)* | 0.05 (0.03–0.09) | 0.09 (0.04–0.13) |
| VEGF (% staining per cell) | 0.41 (0.12–0.74)† | 0.22 (0.12–1.17) | 0.08 (0.04–0.14)† | 0.13 (0.03–0.30) |
*p<0.05; **p<0.01 (between 0-week and 7-week groups).
†p<0.05 (between RCR 0 and GCI 0 weeks).
GCI, glucocorticoid injection; HIF-1α, hypoxia inducible fatcor 1α; PCNA, proliferating cell nuclear antigen; RCR, rotator cuff repair.