Regular ArticleMonoclonal Antibody to Single-Stranded DNA Is a Specific and Sensitive Cellular Marker of Apoptosis
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2019, Journal of Thoracic and Cardiovascular SurgeryCitation Excerpt :This study showed that pirfenidone significantly decreased both of these on lung IRI and reduced apoptotic cells according to the immunohistochemical staining of ssDNA. Staining with anti-ssDNA antibody has been shown to be a specific and sensitive procedure for the detection of apoptotic cells relative to terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling.24 In experiment 2, our results demonstrated that administration of pirfenidone orally by gavage in a rat IRI model increased concentrations of pirfenidone in blood and lung tissue within the experimental period.25-27
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2019, Ecotoxicology and Environmental SafetyCitation Excerpt :The ssDNA antibody is a suitable marker for the early stages of apoptotic cell death associated with DNA damage. Therefore, its immunopositivity is detected in the nuclei of the damaged cells (Frankfurt et al., 1996). A dynamic balance between cell proliferation and apoptotic cell death has been observed throughout spermatogenesis (Shaha et al., 2010).
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