Summary
Blood lactate concentration and the activities of plasma LDH and CK were determined in 13 well-trained middle distance runners after a 400-m sprint. It was found that there is a significant relationship between mean velocity in the 400-m sprint and plasma CK activity (r=−0.56,P<0.05), but the mean sprint velocity did not correlate with peak blood lactate concentration (r=−0.09) or plasma LDH activity (r=−0.40). There was a significant negative correlation between mean sprint velocity and H type LDH isozyme activity (r=−0.66,P<0.05), and a significant positive correlation with M type LDH isozyme activity (r=0.66,P<0.05). These results suggest that the magnitude of enzyme efflux from tissue into blood may be depressed by training, and that in well-trained sprinters plasma CK and LDH isozyme activities may be better indicators of physical training and/or physical performance than peak blood lactate or plasma LDH activities.
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References
Ahlborg B, Brohult J (1967) Immediate and delayed metabolic reactions in well-trained subjects after prolonged physical exercise. Acta Med Scand 182: 41–54
Berg A, Haralambie G (1978) Changes in serum creatine kinase and hexose phosphate isomerase activity with exercise duration. Eur J Appl Physiol 39: 191–201
Booth FW (1981) Enzymatic capacities of skeletal muscle: Effects of different types of training. In: diPrampero PE, Pootmans JR (eds) Physiological chemistry of exercise and training, Medicine and Sport, vol 13. Kager, Basel, pp 153–164
Fowler WM, Gardner GW, Kazerunian HH, Lauvstad WA (1968) The effect of exercise on serum enzymes. Arch Phys Med 49: 554–565
Fujitsuka N, Yamamoto T, Ohkuwa T, Saito M, Miyamura M (1982) Peak blood lactate after short periods of maximal treadmill running. Eur J Appl Physiol 48: 289–296
Garbus J, Highman B, Altland PD (1964) Serum enzymes and lactic dehydrogenase isoenzymes after exercise and training in rats. Am J Physiol 207: 467–472
Helm HJ (1962) A simplified method of demonstrating lactic dehydrogenase isoenzyme in serum. Clin Chim Acta 7: 124–128
Hohorst HJ (1962) L-Lactat Bestimmung mit Lactat-Dehydrogenase und DPN. In: Methods of enzymatic analysis. Verlag Chemie, Weinheim, FRG, pp 266–270
Hunter JB, Critz JB (1971) Effect of training on plasma enzyme levels in man. J Appl Physiol 31: 20–23
Karlsson J, Nordesjö L-O, Jorfeldt L, Saltin B (1972) Muscle lactate, ATP, and CP levels during exercise after physical training in man. J Appl Physiol 33: 199–203
Karlsson J, Sjödin B, Thorstensson A, Hultén B, Frith K (1975) LDH isoenzymes in skeletal muscles of endurance and strength trained athletes. Acta Physiol Scand 93: 150–156
Komi PV, Rusko H, Vos J, Vihko V (1977) Anaerobic performance capacity in athletes. Acta Physiol Scand 100: 107–114
MacDougall JD, Ward GR, Sale DG, Sutton JR (1977) Biochemical adaptation of human skeletal muscle to heavy resistance training and immobilization. J Appl Physiol 43: 700–703
Margaria R, Aghemo P, Rovelli E (1966) Measurement of muscular power (anaerobic) in man. J Appl Physiol 21: 1662–1664
Noakes TD, Cater JW (1982) The responses of plasma biochemical parameters to a 56-km race in novice and experienced ultramarathon runners. Eur J Appl Physiol 49: 179–186
Nuttall FQ, Jones B (1968) Creatine kinase and glutamic oxalacetic transaminase activity in serum: Kinetics of change with exercise and effect of physical conditioning. J Lab Clin Med 71: 847–854
Papadopoulos NM, Leon AS, Bloor CM (1968) Effects of exercise on plasma lactate dehydrogenase and isoenzyme activities in trained and untrained rats. Proc Soc Exp Biol Med 129: 232–234
Rosalki SB (1967) An improved procedure for serum creatine phosphokinase determination. J Lab Clin Med 69: 696–705
Rose LI, Lowe SL, Carroll DR, Wolfson S, Cooper KH (1970) Serum lactate dehydrogenase isoenzyme changes after muscular exertion. J Appl Physiol 28: 279–281
Sanders TM, Bloor CM (1975) Effects of repeated endurance exercise on serum enzyme activities in well-conditioned males. Med Sci Sports 7: 44–47
Sawka MN, Knowlton RG, Miles DS, Crits JB (1979) Post-competition blood lactate concentrations in collegiate swimmers. Eur J Appl Physiol 41: 93–99
Spieckermann PG, Gebhard MM, Nordbeck H (1975) Role of energy metabolism in enzyme retention. A study on isolated perfused canine hearts. Experientia 31: 1046–1047
Suominen H, Heikkinen E, Liesen H, Michel D, Hollman W (1977) Effects of 8 weeks' endurance training on skeletal muscle metabolism in 56–70 year-old sedentary men. Eur J Appl Physiol 37: 173–180
Thomson WHS, Sweeting JC, Hamilton IJD (1975) ATP and muscle enzyme efflux after physical exertion. Clin Chim Acta 59: 241–245
Thorstensson A, Sjödin B, Karlsson J (1975) Enzyme activities and muscle strength after “sprint training” in man. Acta Physiol Scand 94: 313–318
Thorstensson A, Hultén B, Von Döbeln W, Karlsson J (1976) Effect of strength training on enzyme activities and fibre characteristics in human skeletal muscle. Acta Physiol Scand 96: 392–398
Wilkinson JH, Robinson JM (1974) Effect of energy-rich compounds on release of intracellular enzymes from human leukocytes and rat lymphocytes. Clin Chem 20: 1331–1336
Wróblewski F, LaDue JS (1955) Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med 90: 210–213
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Ohkuwa, T., Saito, M. & Miyamura, M. Plasma LDH and CK activities after 400 m sprinting by well-trained sprint runners. Europ. J. Appl. Physiol. 52, 296–299 (1984). https://doi.org/10.1007/BF01015213
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DOI: https://doi.org/10.1007/BF01015213