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Delayed reoxygenation after maximal isometric handgrip exercise in high oxidative capacity muscle

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Abstract

We hypothesized that after maximal short-term isometric exercise, when O2 demand is still high and O2 supply is not fully activated, higher oxidative capacity muscle may exhibit slower muscle reoxygenation after the exercise than low oxidative capacity muscle. Seven healthy male subjects performed a maximal voluntary isometric handgrip exercise for 10 s. The reoxygenation rate after the exercise (Reoxy-rate) in the finger flexor muscle was determined by near infrared continuous wave spectroscopy (NIRcws) while phosphocreatine (PCr) was measured simultaneously by 31P magnetic resonance spectroscopy. Muscle oxygen consumption (muscle O2) and muscle oxidative capacity were evaluated using the rate of PCr resynthesis post-exercise. The forearm blood flow (FBF) index at the end of exercise was measured using NIRcws. There was a significant positive correlation between the Reoxy-rate, which ranged between 0.53% s−1 and 12.47% s−1, and the time constant for PCr resynthesis, which ranged between 17.8 s and 38.3 s (r 2=0.939, P<0.001). At the end of the exercise, muscle O2 exceeded the resting level by approximately 25-fold, while the FBF index exceeded the resting level by only 3-fold on average. The Reoxy-rate closely correlated with muscle O2 (r 2=0.727, P<0.05), but not with the FBF index. Also, the estimated O2 balance (muscle O2 index/FBF index) was negatively correlated with the Reoxy-rate (r 2=0.820, P<0.001). These results support our hypothesis that higher oxidative capacity muscle shows slower muscle reoxygenation after maximal short-term isometric exercise because the Reoxy-rate after this type of exercise may be influenced more by muscle O2 than by O2 supply.

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Acknowledgements

The authors would like to thank the subjects who participated in the study, Dr Sang Yong Bae for his assistance in completing this project, and Toshio Kimura and Gregg Goldstein for all of their help.

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Correspondence to Ryotaro Kime.

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Kime, R., Hamaoka, T., Sako, T. et al. Delayed reoxygenation after maximal isometric handgrip exercise in high oxidative capacity muscle. Eur J Appl Physiol 89, 34–41 (2003). https://doi.org/10.1007/s00421-002-0757-3

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