Abstract
Secretory immunoglobulin A (sIgA) is the major immunoglobulin of the mucosal immune system. Whereas the suppressive effect of heavy training on mucosal immunity is well documented, little is known regarding the influence of hypoxia exposure on sIgA during altitude training. This investigation examined the impact of an 18-day Living high–training low (LHTL) training camp on sIgA levels in 11 (six females and five males) elite cross-country skiers. Subjects from the control group (n=5) trained and lived at 1,200 m of altitude, whereas, subjects from the LHTL group (n=6) trained at 1,200 m, but lived at a simulated altitude of 2,500, 3,000 and 3,500 m (3×6-day, 11 h day−1) in hypoxic rooms. Saliva samples were collected before, after each 6-day phases and 2 weeks thereafter (POST). Salivary sIgA, protein and cortisol were measured. There was a downward trend in sIgA concentrations over the study, which reached significance in LHTL (P<0.01), but not in control (P=0.08). Salivary IgA concentrations were still lower baseline at POST (P<0.05). Protein concentration increased in LHTL (P<0.05) and was negatively correlated with sIgA concentration after the 3,000 and 3,500 m-phase and at POST (P<0.05 all). Cortisol concentrations were unchanged over the study and no relationship was found between cortisol and sIgA. In summary, data were strongly suggestive of a cumulative negative effect of physical exercise and hypoxia on sIgA levels during LHTL training. Two weeks of active recovery did not allow for proper sIgA recovery. The mechanism underlying this depression of sIgA could be mediated by neural factors.
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References
Ambrose CT (1966) Symposium on in vitro studies of the immune responses: III biochemical agents affecting the inductive phase of the secondary antibody response initiated in vitro. Bact Rev 30:408–416
Banister EW, Calvert TW, Savage MV, Bach TM (1975) A systems model of training for athletic performance. Aust J Sports Med 7:7–61
Calbet JA (2003) Chronic hypoxia increases blood pressure and noradrenaline spillover in healthy humans. J Physiol 551:379–386
Carpenter GH, Proctor GB, Ebersole LE, Garrett JR (2004) Secretion of IgA by rat parotid and submandibular cells in response to autonomimetic stimulation in vitro. Int Immunopharmacol 4(8):1005–1014
Chicharro JL, Lucia A, Perez M, Vaquero AF, Urena R (1998) Saliva composition and exercise. Sports Med 26:17–27
Colin J, Marotte H, Crance JP (1999) L’hypoxie d’altitude. In: Colin J, Timbal J (eds) Médecine aérospatiale expansion. Scientifique Publications, Paris, pp 55–74
Durnin JVGA, Womersley J (1974) Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 years to 72 years. Br J Nutr 32:77–97
Fleshner M (2000) Exercise and neuroendocrine regulation of antibody production: protective effect of physical activity on stress-induced suppression of the specific antibody response. Int J Sports Med 21:S14–S19
Gleeson M (2000) Mucosal immunity and respiratory illness in elite athletes. Int J Sports Med 21(Suppl 1):S33–S43
Gleeson M and Pyne DB (2000) Exercise effects on mucosal immunity. Immunol Cell Biol 78:536–544
Gleeson M, McDonald WA, Pyne DB, Cripps AW, Francis JL, Fricker PA, Clancy RL (1999) Salivary IgA levels and infection risk in elite swimmers. Med Sci Sports Exerc 31:67–73
Hucklebridge F, Clow A, Evans P (1998) The relationship between salivary secretory immunoglobulin A and cortisol: neuroendocrine response to awakening and the diurnal cycle. Int J Psychophysiol 31:69–76
Ingjer F, Myhre K (1992) Physiological effects of altitude training on elite male cross-country skiers. J Sports Sci 10:37–47
Klokker M, Kjaer M, Secher NH, Hanel B, Worm L, Kappel M, Pedersen BK (1995) Natural killer cell response to exercise in humans: effect of hypoxia and epidural anesthesia. J Appl Physiol 78(2):709–716
Levine BD, Stray-Gundersen J, Duhaime G et al (1991) Living high–training low: the effect of altitude acclimatization/normoxic training in trained runners [abstract]. Med Sci Sports Exerc 23(Suppl 4):S25
Lopez LR, Cantella RA, Piscoya Z, Colichon AA, Delgado M, Recavarren S (1975) Immunological survey in high altitude: effect on antibody production and the complement system. Ann Sclavo 17(6):769–785
Mathews M, Jia HP, Guthmiller JM, Losh G, Graham S, Johnson GK, Tack BF, McCray PB Jr (1999) Production of beta-defensin antimicrobial peptides by the oral mucosa and salivary glands. Infect Immun 67(6):2740–2745
Mazzeo RS, Dubay A, Kirsch J, Braun B, Butterfield GE, Rock PB, Wolfel EE, Zamudio S, Moore LG (2003) Influence of alpha-adrenergic blockade on the catecholamine response to exercise at 4,300 meters. Metabolism 52(11):1471–1477
McDowell SL, Hughes RA, Hughes RJ, Housh TJ, and Johnson GO (1992) The effect of exercise training on salivary immunoglobulin A and cortisol responses to maximal exercise. Int J Sports Med 13:577–580
Meehan RT (1987) Immune suppression at high altitude. Ann Emerg Med 16(9):974–979
Pedersen BK and Steensberg A (2002) Exercise and Hypoxia: effects on leukocytes and interleukin-6—shared mechanisms? Med Sci Sports Exerc 34:2004–2012
Pedersen BK, Kappel M, Klokker M, Nielsen HB, Secher NH (1994) The immune system during exposure to extreme physiologic conditions. Int J Sports Med 15(Suppl 3):S116–S121
Pyne DV, McDonald WA, Morton DS, Swigget JP, Foster M, Sonnenfeld G, Smith JA (2000) Inhibition of interferon, cytokine, and lymphocyte proliferative responses in elite swimmers with altitude exposure. J Interferon Cytokine Res 20(4):411–408
Ring C, Harrison LK, Winzer A, Carroll D, Drayson M, Kendall M (2000) Secretory immunoglobulin A and cardiovascular reactions to mental arithmetic, cold pressor, and exercise: effects of alpha-adrenergic blockade. Psychophysiology 37(5):634–643
Tharp GD, Barnes M (1990) Reduction of saliva immunoglobulin levels by swim training. Eur J Appl Physiol 60:61–64
Tiollier E, Gomez-Merino D, Burnat P, Jouanin J-C, Bourrilhon C, Filaire E, Guezennec Y, Chennaoui M (2005) Intense training: mucosal immunity and incidence of respiratory infections. Eur J Appl Physiol 93: 421–428
Tomasi TB, Trudeau FB, Czerwinski D (1982) Immune parameters in athletes before and after a strenuous exercise. J Clin Immunol 2:173–178
Welde B, Evertsen F, Von Heimburg E, Medbo JI (2003) Energy cost of free technique and classical cross-country skiing at racing speeds. Med Sci Sports Exerc 35:818–825
Wilber RL (2001) Current trends in altitude training. Sports Med 31(4):249–265
Winzer A, Ring C, Carroll D, Willemsen G, Drayson M, Kendall M (1999) Secretory immunoglobulin A and cardiovascular reactions to mental arithmetic, cold pressor, and exercise: effects of beta-adrenergic blockade. Psychophysiology 36(5):591–601
Zaccaria M, Ermolao A, Bonvicini P, Travain G, Varnier M (2004) Decreased serum leptin levels during prolonged high altitude exposure. Eur J Appl Physiol 92(3):249–253
Acknowledgements
This study was funded by grants from the International Olympic Committee and the French Ministry of Sports. We wish to thank the athletes for their participation in this study and Odile Michaud for analysis of cortisol. The cooperation of the staff of the Centre National de Ski Nordique was appreciated.
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Tiollier, E., Schmitt, L., Burnat, P. et al. Living high–training low altitude training: effects on mucosal immunity. Eur J Appl Physiol 94, 298–304 (2005). https://doi.org/10.1007/s00421-005-1317-4
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DOI: https://doi.org/10.1007/s00421-005-1317-4