Alimentary TractHeat-shock protein 72 protects against oxidant-induced injury of barrier function of human colonic epithelial Caco2/bbe cells☆,☆☆,★
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Cell culture and cell protection
The Caco2/bbe subclone (C2)13 was grown in Dulbecco's modified Eagle medium (DMEM; high glucose, 4.5 g/L) with 10% (vol/vol) fetal bovine serum, 10 μg/mL transferrin, 50 μg/mL streptomycin, and 50 U/mL penicillin. Cells were used between passages 50 and 80. IEC-18 cells were grown in DMEM containing 5% (vol/vol) fetal bovine serum, 0.1 U/mL insulin, and penicillin/streptomycin as above. Cells were used between passages 20 and 27.
The ability of the C2 and IEC-18 cell lines to withstand injury
Monochloramine injury in C2 and IEC-18 cells
Normal diploid intestinal epithelial cells such as the IEC-18 cell line are easily injured by exposure to oxidants, as previously shown.11, 12 In contrast, we had observed that C2 cells were far more resistant to the injurious effects of monochloramine treatment. The difference in cell response to monochloramine induced injury is shown in Figure 1.
Discussion
One of the most important functions of the intestinal mucosa is the maintenance of barrier function, which is not only essential for preventing systemic entry of foreign antigens, bacteria, and bacterial products, but also for other gut functions such as vectorial transport of nutrients, water, and electrolytes. However, mucosal barrier function can be significantly compromised in the setting of inflammatory bowel diseases, particularly by immune and inflammatory mediators such as interferon
References (24)
- et al.
Luminal exposure of oxidants alter colonic absorptive function
Life Sci
(1996) - et al.
Effects of hydrogen peroxide and phorbol myristate acetate on endothelial transport and F-actin distribution
Exp Cell Res
(1995) - et al.
Oxygen free radical injury of IEC-18 small intestinal epithelial cell monolayers
Gastroenterology
(1991) - et al.
Heat shock proteins are differentially expressed in human gastrointestinal cancers
Surg Oncol
(1995) - et al.
Contribution of plasma membrane and endoplasmic reticulum Ca-ATPases to the synaptosomal Cai increase during oxidative stress
Brain Res
(1996) - et al.
Hydroperoxide induced increases in intracellular calcium due to annexin VI translocation and inactivation of plasma membrane Ca-ATPases
J Biol Chem
(1996) - et al.
Ca-mediated stimulation of Cl secretion by reactive oxygen metabolites in human colonic T84 cells
J Clin Invest
(1992) - et al.
Oxidants and conductance of cultured epithelial cell monolayers: inositol phospholipid hydrolysis
Am J Physiol
(1988) - et al.
Oxidants increase paracellular permeability in a cultured epithelial cell line
J Clin Invest
(1985) - et al.
Hyperpermeability of intestinal epithelial monolayers is induced by NO: effect of low extracellular pH
Am J Physiol
(1997)
Tight junctions and the molecular basis for regulation of paracellular permeability
Am J Physiol
Effects of cytochalasin D on occluding junctions of intestinal absorptive cells: further evidence that the cytoskeleton may influence paracellular permeability and junctional charge selectivity
J Cell Biol
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Supported by National Institutes of Health grant DK-47722 (to E.B.C.), the Digestive Disease Center at the University of Chicago DK-42086, National Cancer Institute grant CA-14599 to the University of Chicago Cancer Research Center, and the Gastrointestinal Research Foundation of Chicago.
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Address requests for reprints to: Eugene B. Chang, M.D., Department of Medicine, University of Chicago, MC 6084, 5841 South Maryland Avenue, Chicago, Illinois 60637. e-mail: [email protected]; fax: (773) 702-2281.
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Confocal microscopy was performed in the Al Robin Confocal Core Facility.