ArticlesReduced Levels of Antioxidants in Brains of Apolipoprotein E-Deficient Mice Following Closed Head Injury
Section snippets
Closed Head Injury
Four-month-old apoE-deficient male mice (∼23 g) and a matched group of control mice from the same original parent litter [20]were used in this study. Closed head injury to seven mice in each group was produced under anesthesia as modified by Chen et al. [4]after Shapira et al. [23]. A weight-drop device was employed in which a calibrated weight (333 g) was allowed to fall from a height of 3 cm onto the exposed skull, over the left cervical hemisphere, 1–2 mm lateral to the midline of the
Results
The possibility that the diminished recovery of apoE-deficient mice from closed head injury is related to a diminution in their ability to counteract oxidative damage was examined by cyclic voltammetry. This was pursued by measurements using homogenates of distinct brain areas of injured and noninjured apoE-deficient and control mice. A representative volt-ammogram thus obtained from a cortical homogenate of a control mouse prior to closed head injury is presented in Fig. 1. Cortical
Discussion
This study revealed that prior to head injury, apoE-deficient and control mice have similar levels of antioxidant reducing compounds but that their levels differ markedly following closed head injury. In control mice, closed head injury results in an initial decrease and in a subsequent surge of antioxidant reducing substances, whereas in the apoE-deficient mice, the initial decrease and particularly the subsequent increase are much less pronounced. Below we discuss possible mechanisms that may
Acknowledgements
We thank Prof. D. Lictenberg for his advice and generous help. This work was supported in part by grants to D. M. M. from the Joseph K. & Inez Eichenbaum Foundation, the Simon Revah-Kabelli Fund, the U.S. Israel Binational Foundation, and the Israel Science Foundation, and to L. L. from the Robinowitch Fund.
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