The effect of acute creatine supplementation on stroke quality was investigated during simulated match play. Well-trained tennis players reported to the test center on two occasions. On each occasion they performed the Leuven Tennis Performance Test (LTPT) and a 70 m shuttle run (SHR). During 5 days prior to each test session they received in random order and according to a double-blind cross-over study design either oral creatine supplements (4 x 5 g per day) or placebo. The two experimental periods were separated by a 5-week washout period. Stroke quality was evaluated during the LTPT by means of registration of error rate and measurement of ball velocity and precision of lateral and longitudinal ball placement. Compared with placebo, creatine supplementation did not significantly impact on either power or precision of first and second services, baseline strokes in neutral and defensive rallies, and volleys. Shuttle run time was 19.87 +/- 0.30 sec during placebo versus 19.85 +/- 0.27 sec during creatine treatment. Acute creatine supplementation does not enhance stroke performance or sprint power in match-like conditions in elite tennis players.