This study quantitatively assessed the mechanical reliability and validity of position, torque and velocity measurements of the Biodex System 3 isokinetic dynamometer. Trial-to-trial and day-to-day reliability were assessed during three trials on two separate days. To assess instrument validity, measurement of each variable using the Biodex System 3 dynamometer was compared to a criterion measure of position, torque and velocity. Position was assessed at 5 degrees increments across the available range of motion of the dynamometer. Torque measures were assessed isometrically by hanging six different calibrated weights from the lever arm. Velocity was assessed (30 degrees/s to 500 degrees/s) across a 70 degrees arc of motion by manually accelerating the weighted lever arm. With the exception of a systematic decrease in velocity at speeds of 300 degrees/s and higher, the Biodex System 3 performed with acceptable mechanical reliability and validity on all variables tested.