The purpose of this study was to determine whether cardiorespiratory fitness (CRF) is associated with lower abdominal adiposity in youth. Subjects included healthy 61 African-American and 52 white children and adolescents (age: 8-17 years). Body composition was measured by dual-energy X-ray absorptiometry and computed tomography. CRF (VO(2max)) was assessed using a graded maximal treadmill test. CRF was inversely related (P<0.05) to total adiposity, waist circumference, and visceral and abdominal subcutaneous adipose tissue (AT) independent of race. These findings remained significant (P<0.05) after adjusting for age, gender and pubertal status. Multiple regression analyses revealed that CRF is an independent contributor (P<0.05) of waist circumference, and visceral and abdominal subcutaneous AT after accounting for age, pubertal status, gender and body mass index percentile. Our observation suggest that in youth, CRF is associated with lower visceral and abdominal subcutaneous AT, and reinforces the notion that youth should engage in regular physical activity to improve aerobic fitness and reduce abdominal adiposity.