The purpose of this study was to evaluate the effect of hypoxia (10.8 +/- 0.6% oxygen) on performance of 30 s and 45 s of supramaximal dynamic exercise. Twelve males were randomly allocated to perform either a 30 s or 45 s Wingate test (WT) on two occasions (hypoxia and room air) with a minimum of 1 week between tests. After a 5-min warm-up at 120 W subjects breathed the appropriate gas mixture from a wet spirometer during a 5-min rest period. Resting blood oxygen saturation was monitored with an ear oximeter and averaged 97.8 +/- 1.5% and 83.2 +/- 1.9% for the air (normoxic) and hypoxic conditions, respectively, immediately prior to the WT. Following all WT trials, subjects breathed room air for a 10-min passive recovery period. Muscle biopsies from the vastus lateralis were taken prior to and immediately following WT. Arterialized blood samples, for lactate and blood gases, were taken before and after both the warm-up and the performance of WT, and throughout the recovery period. Open-circuit spirometry was used to calculate the total oxygen consumption (VO2), carbon dioxide production and expired ventilation during WT. Hypoxia did not impair the performance of the 30-s or 45-s WT. VO2 was reduced during the 45-s hypoxic WT (1.71 +/- 0.21 l) compared with the normoxic trial (2.16 +/- 0.26 l), but there was no change during the 30-s test (1.22 +/- 0.11 vs 1.04 +/- 0.17 l for the normoxic and hypoxic conditions, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)