Northern analysis of highly folded goat alpha S1 casein mRNA

Genet Anal. 1996 Jan;12(3-4):143-5. doi: 10.1016/1050-3862(95)00126-3.

Abstract

Northern blotting using glyoxal to denature a highly folded mRNA, such as goat alpha S1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern / methods*
  • Caseins / genetics*
  • Formaldehyde / pharmacology
  • Glyoxal / pharmacology*
  • Goats
  • Methylmercury Compounds / pharmacology
  • Nucleic Acid Conformation
  • Nucleic Acid Denaturation
  • RNA, Messenger / analysis*

Substances

  • Caseins
  • Methylmercury Compounds
  • RNA, Messenger
  • Formaldehyde
  • Glyoxal
  • methylmercury hydroxide