The effect of heavy, long duration aerobic exercise on low density lipoprotein (LDL) susceptibility to oxidation and on distribution of LDL subfractions was studied. Six well-trained runners, previously fasted, ran continuously for 4 h. Controlled intake of liquid and food was permitted during exercise. Total plasma and LDL triglyceride increased significantly. LDL susceptibility to oxidation, measured as conjugated dienes formation, was modified significantly (P < or = 0.05) after running (14% reduction in lag phase time, and 8% increase in maximal curve slope). The percentage of electronegative LDL form (named LDLB) also increased significantly (P < or = 0.05) after exercise both basally (from 7.3% to 11%) and after 2h of induced oxidation (from 40.6% to 52.3%). Neither LDL susceptibility to oxidation nor increase of LDLB was statistically associated with food consumed during the race or modifications of triglycerides suggesting that this effect was due to exercise rather than food-related. The pattern of LDL subfractions was type A in all athletes before and after running. The oxidative LDL changes, seen in exercise conditions similar to those of hard training or competition, demonstrated an unfavourable effect of very intense exercise on lipoprotein metabolism.