New tools provide new insights in NMR studies of protein dynamics
A Mittermaier, LE Kay - science, 2006 - science.org
There is growing evidence that structural flexibility plays a central role in the function of
protein molecules. Many of the experimental data come from nuclear magnetic resonance …
protein molecules. Many of the experimental data come from nuclear magnetic resonance …
THE USE OF 2H, 13C, 15N MULTIDIMENSIONAL NMR GTO STUDY THE STRUCTURE AND DYNAMICS OF PROTEINS
KH Gardner, LE Kay - Annual review of biophysics and …, 1998 - annualreviews.org
▪ Abstract During the past thirty years, deuterium labeling has been used to improve the
resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most …
resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most …
Biological underpinnings for lifelong learning machines
Biological organisms learn from interactions with their environment throughout their lifetime.
For artificial systems to successfully act and adapt in the real world, it is desirable to similarly …
For artificial systems to successfully act and adapt in the real world, it is desirable to similarly …
[BOOK][B] Who wrote the book of life?: A history of the genetic code
LE Kay - 2000 - books.google.com
This is a detailed history of one of the most important and dramatic episodes in modern
science, recounted from the novel vantage point of the dawn of the information age and its …
science, recounted from the novel vantage point of the dawn of the information age and its …
Backbone dynamics of a free and a phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation
NA Farrow, R Muhandiram, AU Singer, SM Pascal… - Biochemistry, 1994 - ACS Publications
Revised Manuscript Received March 10, 19949 abstract: The backbone dynamics of the C-
terminal SH2 domain of phospholipase C7I have been investigated. Two forms of the …
terminal SH2 domain of phospholipase C7I have been investigated. Two forms of the …
Backbone dynamics of proteins as studied by nitrogen-15 inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease
Revised Manuscript Received September 7, 1989 abstract: This paper describes the use of
novel two-dimensional nuclear magnetic resonance (NMR) pulse sequences toprovide …
novel two-dimensional nuclear magnetic resonance (NMR) pulse sequences toprovide …
Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity
Figure 1. Pulse sequence of the sensitivity enhanced gradient'H-15N HSQC experiment.
Narrow pulses represent a flip angle of 90 with wider pulses representing a flip angle of 180 …
Narrow pulses represent a flip angle of 90 with wider pulses representing a flip angle of 180 …
Three-dimensional triple-resonance NMR spectroscopy of isotopically enriched proteins
Four new and complementary three-dimensional triple-resonance experiments are
described for obtaining complete backbone 1 H, 13 C, and 15 N resonance assignments of …
described for obtaining complete backbone 1 H, 13 C, and 15 N resonance assignments of …
Intrinsic dynamics of an enzyme underlies catalysis
EZ Eisenmesser, O Millet, W Labeikovsky… - Nature, 2005 - nature.com
A unique feature of chemical catalysis mediated by enzymes is that the catalytically reactive
atoms are embedded within a folded protein. Although current understanding of enzyme …
atoms are embedded within a folded protein. Although current understanding of enzyme …
[PDF][PDF] A novel approach for sequential assignment of 1H, 13C, and 15N spectra of larger proteins: Heteronuclear triple-resonance three-dimensional NMR …
A novel approach is described for obtaining sequential assignment of the backbone'H, I3C,
and 15N resonances of larger proteins. The approach is demonstrated for the protein …
and 15N resonances of larger proteins. The approach is demonstrated for the protein …